甘草泻心汤对溃疡性结肠炎大鼠水通道蛋白3、4的影响  

Effects of Gancao Xiexin decoction on aquaporin 3 and 4 in rats with ulcerative colitis

作　　者：雷娜 雷兰平 吴容 权娜娜 唐学贵[4] LEI Na;LEI Lanping;WU Rong;QUAN Nana;TANG Xuegui(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China.;the Second Xiangya Hospital of Central South University,Changsha 410013.;the People’s Hospital of Pengzhou,Pengzhou 611930.;Affiliated Hospital of North Sichuan Medical College,Nanchong 637000)

机构地区：[1]成都中医药大学,成都611137 [2]中南大学湘雅二医院,长沙410013 [3]彭州市人民医院,四川彭州611930 [4]川北医学院附属医院,四川南充637000

出　　处：《中国比较医学杂志》2023年第8期47-54,共8页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(82074429)。

摘　　要：目的探讨甘草泻心汤对溃疡性结肠炎(UC)大鼠水通道蛋白3、4的影响。方法清洁级SD大鼠32只,抽取8只作为空白组,余下24只采用4%葡聚糖硫酸钠复制UC模型,模型成功后随机分为模型组,西药组(美沙拉嗪缓释颗粒),中药组(甘草泻心汤),每组8只,持续灌胃2周,每天1次。检测并记录体质量、疾病活动指数、结肠长度、结肠黏膜损伤指数、组织病理学改变和结肠组织学损伤指数,血清肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、IL-6,结肠水通道蛋白3(AQP3)、水通道蛋白4(AQP4)蛋白和mRNA。结果与空白组相比,模型组和给药组血清TNF-α、IL-1β、IL-6浓度升高(P<0.05);模型组和给药组结肠AQP3、AQP4蛋白表达降低(P<0.05);模型组、给药组结肠AQP3 mRNA表达和模型组、西药组结肠AQP4 mRNA表达降低(P<0.05)。中药组结肠AQP4 mRNA表达与空白组相比无统计学差异(P>0.05)。与模型组相比,给药组血清TNF-α、IL-1β、IL-6浓度降低(P<0.05);中药组血清TNF-α、IL-1β、IL-6浓度比西药组更低(P<0.05);给药组结肠AQP3、AQP4蛋白和mRNA表达升高(P<0.05);中药组结肠AQP4蛋白表达比西药组更高(P<0.05);中药组结肠AQP3、AQP4 mRNA表达比西药组更高(P<0.05)。结论甘草泻心汤能有效改善UC大鼠体质量、疾病活动指数、结肠黏膜损伤指数、组织学损伤指数等情况,其可能通过改善结肠AQP3、AQP4蛋白和mRNA表达,调节血清TNF-α、IL-1β、IL-6水平,推测这些可能是甘草泻心汤治疗UC的效应机制。Objective To explore the effects of Gancao Xiexin decoction(GXD)on aquaporin(AQP)3 and AQP4 in rats with ulcerative colitis(UC).Methods Thirty-two clean SD rats,eight SD rats were used as the blank group,the remaining 24 rats were used to replicate the UC model with 4%dextran sulfate sodium.After the model was successful,the rats were divided into model,Western medicine(slow-release mesalazine granules),and Chinese medicine(Gancao Xiexin decoction)groups,eight rats in each group and then gavaged continuously for 2 weeks once a day.Body mass,disease activity index,colon length,colonic mucosa damage index,histopathological changes,histological damage index,serum tumor necrosis factor-α(TNF-α),interleukin-(IL)-1β,IL-6 and colonic AQP3 and AQP4 were assessed.Results Compared with the blank group,serum levels of TNF-α,IL-1β,and IL-6 in model and administration groups were increased(P<0.05),colonic AQP3 and AQP4 levels in model and administration groups were decreased(P<0.05),and colonic AQP3 mRNA levels in model and administration groups,and AQP4 mRNA levels in model and Western medicine groups were declined(P<0.05).There was no remarkable difference in mRNA expression of colonic AQP4 between Chinese medicine and blank groups(P>0.05).Compared with the model group,serum levels of TNF-α,IL-1β,and IL-6 in Western and Chinese medicine groups were decreased(P<0.05),serum levels of TNF-α,IL-1βand IL-6 in the Chinese medicine group were lower than those in the Western medicine group(P<0.05),colonic AQP3 and AQP4 protein and mRNA levels in administration groups were increased(P<0.05),the colonic AQP4 protein level in the Chinese medicine group was higher than that in the Western medicine group(P<0.05),and colonic AQP3 and AQP4 mRNA levels in the Chinese medicine group were higher than those in the Western medicine group(P<0.05).Conclusions GXD effectively improves the body weight,disease activity index,colonic mucosa damage index,and histological damage index of UC rats,which may regulate serum TNF-α,IL-1β,and IL-6

关 键 词：甘草泻心汤 溃疡性结肠炎 水通道蛋白3 水通道蛋白4 炎症因子 

分 类 号：R-33[医药卫生]