草地贪夜蛾高致病力绿僵菌菌株的筛选与鉴定  

Screening and identification of highly pathogenic strains of Metarhizium against Spodoptera frugiperda

作　　者：赵建伟[1] 丁雪玲[1] 郑宇[1] 赵金凤 姚凤銮[1] 卢学松[1] 何玉仙[1] ZHAO Jian-Wei;DING Xue-Ling;ZHENG Yu;ZHAO Jin-Feng;YAO Feng-Luan;LU Xue-Song;HE Yu-Xian(Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fujian Engineering Research Center for Green Pest Management,Fuzhou 350013,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建省农业科学院植物保护研究所,福建省作物有害生物监测与治理重点实验室,福建省作物有害生物绿色防控工程研究中心,福州350013 [2]福建农林大学植物保护学院,福州350002

出　　处：《环境昆虫学报》2023年第4期1111-1118,共8页Journal of Environmental Entomology

基　　金：福建省属公益类科研院所基本科研专项(2020R1024009);福建省自然科学基金(2021J01477);福建省科技厅引导性项目(2020N0026);福建省农科院创新团队项目(CXTD2021027);“5511”协同创新工程(XTCXGC2021011,2021017)。

摘　　要：为获取对草地贪夜蛾Spodoptera frugiperda具有高致病力的生防真菌,从福建省不同地区分离得到8株寄主为鳞翅目和半翅目幼虫僵虫的绿僵菌Metarhizium,采用浸渍法测定了其对草地贪夜蛾2龄幼虫和蛹的致病力,并根据形态学和分子生物学方法对高致病力菌株进行种类鉴定。结果表明,8株绿僵菌菌株对草地贪夜蛾2龄幼虫和蛹均表现出不同程度的致病力,其中菌株FJMR2和FJXY7表现出较强的致病力。在5×10^(7)个/mL孢子浓度下,FJMR2和FJXY7对草地贪夜蛾2龄幼虫的致死率分别为88.76%和82.13%,对蛹的致死率分别为86.57%和84.00%;对草地贪夜蛾2龄幼虫的LT 50分别为4.81 d和4.93 d,对蛹的LT 50分别为4.94 d和4.83 d。经鉴定菌株FJMR2和FJXY7均为莱氏绿僵菌Metarhizium rileyi。本研究获得2株对草地贪夜蛾2龄幼虫和蛹具有高致病力的莱氏绿僵菌菌株,在草地贪夜蛾的生物防治中具有较大应用潜力。Virulence of eight Metarhizium strains as their hosts isolated from the larvae of Lepidoptera and Hemiptera was evaluated against the 2 nd instar larvae and pupae of Spodoptera frugiperda by using dipping method,and the strains with strong virulence were identified according to morphological and molecular biological methods.The results indicated that the eight isolates showed different degrees of pathogenicity to the 2 nd instar larvae and pupae of S.frugiperda,among which strain FJMR2 and FJXY7 showed strong virulence.When the concentration of strain FJMR2 and FJXY7 reached 5×10^(7) spores/mL,the corrected mortality to the 2 nd instar larvae of S.frugiperda were 88.76%and 82.13%respectively,and the corrected mortality to pupae were 86.57%and 84.00%respectively;LT 50 values of strain FJMR2 and FJXY7 were 4.81 and 4.93 d to the 2 nd instar larvae,and 4.94 and 4.83 d to the pupae,respectively.The strain FJMR2 and FJXY7 were identified as Metarhizium rileyi based on morphological characteristics and rDNA ITS sequence analysis.In this study,two strains of M.rileyi with strong virulence to the 2 nd instar larvae and pupae of S.frugiperda were successfully screened,which showed great potential for the biological control of S.frugiperda.

关 键 词：草地贪夜蛾 绿僵菌 致病力 种类鉴定 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]