工业酶催化剂构建与反应过程强化新技术  

Construction of industrial enzyme catalysts and new technologies for strengthening reaction processes

作　　者：栾鹏仟 冯玉晓 卢滇楠 蒋国强 刘铮 戈钧 LUAN Pengqian;FENG Yuxiao;LU Diannan;JIANG Guoqiang;LIU Zheng;GE Jun(Key Laboratory for Industrial Biocatalysis of the Ministry of Education,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学化学工程系工业生物催化教育部重点实验室,北京100084

出　　处：《生物加工过程》2023年第5期532-540,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2021YFC2102800)。

摘　　要：酶催化由于温和条件下的高选择性推动了化学工业可持续发展。然而,稳定性差和催化反应类型匮乏是天然酶在实际工业应用中面临的两大关键挑战。近年来,随着化工、生物、化学和材料等领域的深度融合,开发高稳定、高重复使用性的固定化酶和构建拓展催化反应类型的酶金属复合催化剂,分别为解决酶催化实际工业应用中稳定性差和催化反应类型受限的问题提供了新的方案。本文主要综述近年来在构建固定化酶和酶金属复合催化剂领域取得的一些进展,重点介绍酶纳米凝胶、酶无机纳米晶体复合物和酶金属复合催化剂方面的研究进展,并探讨该领域在未来发展中所面临的问题与挑战。Enzymatic catalysis promotes the sustainable development of chemical industry due to its high selectivity under mild conditions.However,poor stability and limited catalytic reaction types are two major challenges faced by natural enzymes in practical industrial applications.In recent years,with the deep integration of chemical engineering,biology,chemistry,material science and other fields,significant accomplishment was achieved with regard to the development of highly stable and reusable immobilized enzymes and the construction of enzyme-metal hybrid catalysts.In this review,we described the recent research progress of immobilized enzymes and enzyme-metal hybrid catalysts,and summarized the research achievements in the construction of enzyme nanogel,enzyme-inorganic nanocrystal complexes and enzyme-metal hybrid catalysts.In the end,we presented the problems and challenges for the future development in this field.

关 键 词：生物催化 固定化酶 酶金属复合催化剂 反应过程强化 工业酶催化剂 

分 类 号：Q814[生物学—生物工程]