EZH2能够调节MDA-MB-231三阴性乳腺癌细胞的增殖,周期,凋亡和侵袭  

EZH2 regulates the proliferation,cycle,apoptosis and invasion of MDA-MB-231 triple negative breast cancer cells

作　　者：张瑞山[1] 李翔[1] 刘壮凯[1] 张鑫丰[1] 赵钊[1] 王玉颖[1] 徐宏[1] ZHANG Rui-shan;LI Xiang;LIU Zhuang-kai;ZHANG Xin-feng;ZHAO Zhao;WANG Yu-ying;XU Hong(Department of Breast,Cancer Hospital of China Medical University,Liaoning Province Cancer Hospital,Shenyang 110042,China)

机构地区：[1]中国医科大学肿瘤医院、辽宁省肿瘤医院乳腺科,辽宁沈阳110042

出　　处：《解剖科学进展》2023年第3期267-269,277,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金指导计划(20180550842)。

摘　　要：目的 分析EZH2对MDA-MB-231三阴性乳腺癌细胞生物学行为的调节能力。方法 通过质粒转染的方法过表达和沉默MDA-MB-231三阴性乳腺癌细胞中的EZH2;通过MTT分析EZH2对细胞增殖的影响;通过流式细胞检测分析EZH2对细胞凋亡和周期的影响;通过Transwell分析EZH2对细胞侵袭能力的影响。结果 EZH2的过表达能够促进MDA-MB-231三阴性乳腺癌细胞的增殖,增加处于分裂期细胞的比例,抑制细胞凋亡,促进细胞侵袭;EZH2的沉默能够抑制MDA-MB-231三阴性乳腺癌细胞的增殖,减少处于分裂期细胞的比例,促进细胞凋亡,抑制细胞侵袭。结论 EZH2具有调节MDA-MB-231三阴性乳腺癌细胞增殖、周期、凋亡和侵袭的能力。Objective To analyze the regulatory ability of EZH2 on the biological behavior of MDA-MB-231 triple-negative breast cancer cells.Methods Overexpress and silence EZH2 in MDA-MB-231 triple-negative breast cancer cells by plasmid transfection.The effect of EZH2 on cell proliferation was analyzed by MTT method.The effect of EZH2 on cell apoptosis and cycle was analyzed by flow cytometry.The impact of EZH2 on cell invasion was analyzed by Transwell.Results Overexpression of EZH2 can promote the proliferation of MDA-MB-231 triple-negative breast cancer cells,increase the proportion of cells in the dividing phase,inhibit cell apoptosis,and promote cell invasion.Silencing of EZH2 can inhibit the proliferation of MDA-MB-231 triple-negative breast cancer cells,reduce the proportion of cells in the dividing phase,promote cell apoptosis and inhibit cell invasion.Conclusion EZH2 has the ability to regulate the proliferation,cycle,apoptosis and invasion of MDA-MB-231 triple-negative breast cancer cells.

关 键 词：EZH2 三阴性乳腺癌 增殖 周期 凋亡 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]