机械拉伸下乳腺导管内癌组织的Mueller矩阵表征  

Mueller Matrix Characterization of Intraductal Cancerous Tissue of Breast Under Mechanical Stretching

作　　者：褚金奎[1] 辛本达 陈永台 黄波[2,3,4] 丁晓涵[1] Chu Jinkui;Xin Benda;Chen Yongtai;Huang Bo;Ding Xiaohan(School of Mechanical Engineering,Dalian University of Technology,Dalian 116023,Liaoning,China;Liaoning Cancer Hospital&Institute,Shenyang 110042,Liaoning,China;Cancer Hospital,China Medical University,Shenyang 110042,Liaoning,China;Cancer Hospital,Dalian University of Technology,Shenyang 110042,Liaoning,China)

机构地区：[1]大连理工大学机械工程学院,辽宁大连116023 [2]辽宁省肿瘤医院,辽宁沈阳110042 [3]中国医科大学肿瘤医院,辽宁沈阳110042 [4]大连理工大学附属肿瘤医院,辽宁沈阳110042

出　　处：《中国激光》2023年第15期131-139,共9页Chinese Journal of Lasers

基　　金：国家自然科学基金(52175265,52275281,51621064);中央高校基本科研业务费专项基金(DUT21ZD101,DUT21GF308)。

摘　　要：相较于传统非偏振光学表征方法,Mueller矩阵成像偏振(MMIP)法可以表征丰富的生物组织微观结构,在癌症病理诊断方面有着巨大的应用潜力.研究了机械拉伸下不同生物组织结构的MMIP表征,在MMIP系统的基础上增加机械拉伸模块,通过机械拉伸改变生物组织的光学特性.采用Mueller矩阵极化分解方法提取了可以表征介质基本偏振特性的2个表征参数,即二向色性(D)和散射退偏(△).以乳腺导管内癌组织及正常乳腺组织为研究对象,分析了不同组织结构之间表征参数的平均值差值的变化趋势及其变化率在机械拉伸作用下的变化规律.结果表明:在机械拉伸作用下,不同组织结构之间的D参数平均值差值呈减小趋势,差值变化率最大可达7.9%,△参数平均值差值亦呈现减小趋势,差值变化率最大可达12.7%.研究结果为基于偏振成像的癌症病理诊断提供了参考.Objective In recent years,the cases of cancer have increased making it one of the most deadly diseases with breast cancer being the most prevalent in women.An early and effective diagnosis of breast cancer is crucial to the recovery of patients.Compared with traditional optical techniques using unpolarized light,polarization imaging can bring to light the rich microstructure of biological tissues and has the advantages of easy operation,fast detection,and no need for sample staining.Polarization imaging has shown great potential for application in biological tissue analysis and has been applied to the study of pathological diagnosis of cancer.However,during cancerous tissue testing,the mechanical action such as sectioning is required for pathological section preparation.The optical properties of cancerous tissues are affected by mechanical interactions,which generate strains that alter the interrelationship of the cancerous tissue microstructure,leading to changes in the scattering microenvironment and ultimately affecting the inversion of the polarization parameters.Therefore,it is important to investigate the effect of mechanical action such as stretching on the optical properties of cancerous tissues using polarization imaging as a tool in the pathological diagnosis of cancer.Methods The tissue section is elongated to a certain extent in the vertical feed direction because of the compression of the blade on the tissue in the feed direction during the preparation of the tissue section.Based on this concept,this study is conducted to investigate the effect of mechanical stretching on the Mueller matrix characterization of intraductal cancerous breast tissue.As the magnitude of these mechanical actions is small,a system is developed that enables achieving mild mechanical stretches.The method uses the Mueller matrix polar decomposition method to determine two characterization parameters that can characterize the fundamental polarization properties of a medium:dichroism(D)and scattering depolarization(Δ).The mean

关 键 词：医用光学 偏振成像探测 Mueller矩阵参数 乳腺导管内癌 机械拉伸 

分 类 号：O436.3[机械工程—光学工程]