肠器官芯片构建技术及应用研究进展  

Development and application of intestine⁃on⁃a⁃chip technology

作　　者：胡亮 裴雪涛 李艳华 HU Liang;PEI Xuetao;LI Yanhua(Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;South China Research Centerfor Stem Cell&Regenerative Medicine,South China Institute of Biomedicine,Guangzhou 510005,China)

机构地区：[1]军事科学院军事医学研究院辐射医学研究所,北京100850 [2]华南生物医药研究院华南干细胞与再生医学研究中心,广州510005

出　　处：《军事医学》2023年第8期561-565,共5页Military Medical Sciences

基　　金：国家自然科学基金面上项目(82270132,81872553);国家自然科学基金青年科学基金项目(82200122)。

摘　　要：肠器官芯片是近年来新兴的体外肠道仿生模型,相对于传统的肠上皮细胞静态培养模型而言,肠芯片具有与体内肠组织相似的形态结构、可模拟肠组织微生理环境、可规模化定制并实现高通量药物筛选等优势,已广泛应用于肠道疾病模型制备、新药筛选和测试等研究领域。肠芯片建立于肠上皮细胞及其他多种类型细胞共培养的体系之上,并结合微工程技术构建肠道微绒毛、肠褶皱等肠道三维仿生结构,还通过使用微流控装置来模拟肠组织内的血液流动和生理蠕动等生物力学参数,使其具有天然肠组织相似的微生理特征并受到生物力学调控。该文主要从细胞共培养技术、微流控技术、微工程技术等方面对肠器官芯片构建技术的发展进行综述,同时总结了其在肠相关疾病模型模拟、药物筛选和测试等方面的应用。The intestine⁃on⁃a⁃chip is a cutting⁃edge and in vitro research model of human intestines that has gained much attention in recent years.In comparison to traditional static culture models of intestinal epithelial cells,the intestine⁃on⁃a⁃chip offers numerous advantages.It accurately mimics the morphological structure of the intestine in vivo,allowing for the simulation of the microphysiological environment in intestinal tissues.Furthermore,it can be customized on a large scale and enables high⁃throughput drug screening.The intestine⁃on⁃a⁃chip has widespread applications in various areas,including the development of intestinal disease models,as well as drug screening and testing.The technology behind the intestine⁃on⁃a⁃chip is based on a co⁃culture system of intestinal epithelial cells and other cell types.Such chips can not only construct three⁃dimensional structures such as intestinal microvilli and folds in combination with microengineering techniques,but also replicate the intricacies of the intestine.Microfluidic devices are employed to simulate biomechanical parameters like blood flow and physiological peristalsis within the intestinal tissues,thus giving such chips microphysio⁃logical characteristics akin to those of natural intestinal tissues and subjecting it to biomechanical regulation.This review provided an overview of the advances in intestine⁃on⁃a⁃chip technology,focusing on cell co⁃culture techniques,microfluidic technology,and microengineering techniques.Furthermore,the application of this technology in intestinal disease modeling,and drug screening and testing were summarized.

关 键 词：肠 人工生物器官 细胞共培养 微工程技术 疾病模型 药物临床前评价 

分 类 号：R574[医药卫生—消化系统]