超活化血小板裂解液和骨髓间充质干细胞联合应用对大鼠上颌快速扩弓后前腭缝骨改建影响的实验研究  

An experimental study on effects of the combination of super-activated platelet lysate and bone marrow mesenchymal stem cells on bone remodeling of the premaxillary suture after rapid maxillary expansion in rats

作　　者：李佳楠 刘潇遥 刘洋[2] 芦慧颖 王培军 张怡 LI Jianan;LIU Xiaoyao;LIU Yang;LU Huiying;WANG Peijun;ZHANG Yi(Department of Orthodontics,School of Stomatology,Harbin Medical University,Department of Orthodontics,the First Affiliated Hospital of Harbin Medical University,Harbin 150000)

机构地区：[1]哈尔滨医科大学口腔医学院正畸科•哈尔滨医科大学附属第一医院口腔正畸科,150000 [2]哈尔滨医科大学附属第四医院松北分院口腔科 [3]天晴干细胞股份有限公司

出　　处：《现代口腔医学杂志》2023年第4期250-255,267,共7页Journal of Modern Stomatology

基　　金：白求恩公益基金会恒学精进-医学研究项目(2021HX032)。

摘　　要：目的研究超活化血小板裂解液(super-activated platelet lysate,sPL)、骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)及二者联合应用对大鼠上颌快速扩弓后前腭缝骨改建的影响。方法16只6周龄雄性SD大鼠随机分为4组:对照组(A组);sPL注射组(B组);BMSCs注射组(C组);BMSCs+sPL注射组(D组)。建立大鼠上颌快速扩弓模型,扩弓7天后进入保持期,并按分组进行干预。保持21天后处死所有大鼠,通过MicroCT和组织学染色对前腭缝进行骨质分析和组织形态学观察。结果D组大鼠前腭缝宽度相比A组明显减小(P<0.05),骨小梁分离度(Tb.Sp)明显降低(P<0.01),骨小梁数目(Tb.N)明显增多(P<0.05),骨体积分数(BV/TV)显著高于A组和C组(P<0.05)。与A组大鼠相比,B组大鼠BV/TV增高,Tb.Sp降低,差异均有统计学意义(P<0.05)。C组大鼠Micro-CT各项测量指标与A组相比无统计学差异(P>0.05)。组织学观察显示,B、C、D组大鼠前腭缝处骨改建较A组更活跃,D组大鼠单位骨小梁周长成骨细胞数明显高于A组和C组(P<0.05)。结论sPL单独应用或者BMSCs+sPL联合应用均能促进大鼠上颌快速扩弓后前腭缝骨改建,促进骨质形成。Objective To evaluate the effects of super-activated platelet lysate(sPL),bone marrow mesenchymal stem cells(BMSCs)and their combination on bone remodeling of the premaxillary suture after rapid maxillary expansion in rats.Methods Sixteen 6-week-old male SD rats were randomly divided into four groups:control group(group A);sPL injection group(group B);BMSCs injection group(group C);BMSCs+sPL injection group(group D).A rapid maxillary expansion animal model was established.After 7 days of expansion,all rats entered the retention period and interventions were performed by subgroup.All rats were sacrificed after 21 days of retention,and Micro-CT analyses and hematoxylin and eosin(HE)staining were conducted to evaluate the bone structure and morphological changes in the premaxillary suture.Results The premaxillary suture width was significantly reduced in group D compared with group A(P<0.05),the trabecular separation(Tb.Sp)were significantly lower(P<0.01),and the trabecular number(Tb.N)were significantly more(P<0.05).The bone volume fraction(BV/TV)in group D rats was significantly higher than that in groups A and C(P<0.05).Compared with rats in group A,rats in group B had higher BV/TV and lower Tb.Sp,all with statistically significant differences(P<0.05).There was no statistical difference in all measurements of Micro-CT in group C rats compared with group A(P>0.05).Histological observations showed that the bone remodeling of the premaxillary suture was more active in rats in groups B,C and D than in group A.The osteoblast number/unit trabecular perimeter of rats in group D was significantly higher than that in group A and C(P<0.05).Conclusion Both sPL alone and the combination of BMSCs and sPL promoted bone remodeling of the premaxillary suture after rapid maxillary expansion in rats.

关 键 词：上颌快速扩弓 骨改建 超活化血小板裂解液 骨髓间充质干细胞 

分 类 号：R783.5[医药卫生—口腔医学]