超声辅助大孔树脂技术富集荆芥穗中总黄酮和总酚的工艺研究  

Research on process of enriching total flavonoids and total phenols in Schizonepetae Spica by ultrasound-assisted macroporous resin technology

作　　者：王冰倩 周瑾欣 魏星 于生[1,2] 张丽 吴啟南[1,2] 单鸣秋 WANG Bing-qian;ZHOU Jin-xin;WEI Xing;YU Sheng;ZHANG Li;WU Qi-nan;SHAN Ming-qiu(Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中草药》2023年第17期5541-5549,共9页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81973435);江苏省中医药科技发展计划项目(MS2022005)。

摘　　要：目的通过超声辅助大孔树脂对荆芥穗Schizonepetae Spica水提物中总黄酮和总酚类化合物进行富集并对比处理前后化合物含量及活性变化。方法从7种不同类型树脂中筛选超声辅助最佳树脂型号,在吸附动力学和吸附热力学研究下,对超声温度、功率、上样质量浓度及解吸溶剂百分比等影响因素进行工艺优化。测定了优化处理前后各成分含量及活性变化。结果动力学和热力学的研究表明,荆芥穗总黄酮适用于Langmuir模型,且吸附过程为自发吸热熵增,总酚则为自发放热熵减并适用于Freundlich模型,据此不同的吸附机制优化了2类成分同时富集的最佳工艺,在超声辅助AB-8大孔树脂,温度25℃、时间2.5 h,功率150 W,上样质量浓度0.1 g/mL,70%乙醇解吸附的过程下,荆芥穗水提物中总黄酮和总酚类化合物含量增加2~3倍,其单体成分的含量及抗氧化和神经氨酸酶抑制活性均显著增加。结论超声辅助大孔树脂技术为荆芥穗总黄酮和总酚类成分的有效可行富集技术。Objective To enrich the total flavonoids and total phenolic in the aqueous extracts of Jingjiesui(Schizonepetae Spica,SS)by ultrasonic-assisted macroporous resin,and compare with the changes of compound content and activity before and after treatment.Methods The best ultrasound-assisted resin model was screened from seven different types of resins,and the influencing factors such as ultrasonic temperature,power,sample concentration and percentage of solvent desorption were optimized under the study of adsorption dynamics and thermodynamics.The changes in content and activity of each compound before and after the enrichment treatment were evaluated in terms of DPPH radical scavenging capacity and neuraminidase inhibition capacity.Results The study of kinetics and thermodynamics showed that the total flavonoids is suitable for the Langmuir model,the adsorption process is spontaneous endothermic entropy increase,the total phenol is spontaneous exothermic,and the optimal process for the simultaneous enrichment of the two types of components is optimized through this adsorption mechanism.In the process of ultrasound-assisted AB-8 macroporous resin(25℃for temperature,2.5 h for time,150 W for power,0.1 g/mL for sample concentration,70%ethanol for desorption solvent),the content of total flavonoids and total phenolic compounds in the water extract of SS increased 2—3 times,and the content of monomer components and its antioxidant and neuraminidase inhibitory activities were increased significantly.Conclusion Ultrasonicassisted macroporous resin technique is a effective enrichment technique of total flavonoids and total phenolic components in SS.

关 键 词：荆芥穗 大孔树脂 超声辅助 总黄酮 总酚 吸附动力学 吸附热力学 神经氨酸酶 橙皮苷 木犀草苷 迷迭香酸 木犀草素-7-O-葡萄糖醛酸苷 

分 类 号：R283.6[医药卫生—中药学]