老香黄多糖的分离纯化及其体外胃黏膜保护活性研究  

Studies on Isolation,Purification and Gastric Mucosal Protective Activity in Vitro of Laoxianghuang Polysaccharide

作　　者：阳丹 蔡舒 彭成海 陈树喜 周爱梅[1] YANG Dan;CAI Shu;PENG Chenghai;CHEN Shuxi;ZHOU Aimei(Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,College of Food Science,South China Agricultural University,Guangzhou 510642,China;Guangdong Zhancui Food Co.,Ltd.,Chaozhou 515634,China;Huanong(Chaozhou)Food Research Institute Co.,Ltd.,Chaozhou 521021,China)

机构地区：[1]华南农业大学食品学院,广东省功能食品活性物重点实验室,广东广州510642 [2]广东展翠食品股份有限公司,广东潮州515634 [3]华农(潮州)食品研究院有限公司,广东潮州521021

出　　处：《食品工业科技》2023年第19期440-448,共9页Science and Technology of Food Industry

基　　金：潮州市科技项目(2020ZX03)。

摘　　要：本文以老香黄多糖为研究对象,探究其胃黏膜保护活性。首先采用热水浸提法提取老香黄多糖,进而通过Sevag试剂脱蛋白、DEAE-52纤维柱和CL-6B琼脂糖凝胶柱进行多糖的分离纯化,最后采用乙醇损伤人胃黏膜上皮细胞(Human gastric mucosal epithelial cells,GES-1)细胞模型及GES-1细胞划痕损伤模型探究其体外胃黏膜保护活性。结果表明,老香黄多糖经纯化后共得到6个组分,分别为PFCP、PFCP-1、PFCP-2、PFCP-3、PFCP-2-1、PFCP-2-2。在乙醇损伤GES-1细胞实验中,老香黄多糖各分离纯化组分均能提高细胞活性,增强细胞超氧化物歧化酶(SOD)、过氧化氢酶(CAT)酶活,减少丙二醛(MDA)含量,从而增强细胞抗氧化能力,减轻黏膜损伤。其中PFCP-2-1(50μg/mL)提高SOD酶活和降低MDA含量效果最佳,PFCP-2-1(5μg/mL)提高CAT酶活效果最佳,与模型组相比均有显著性(P<0.05),且优于替普瑞酮阳性药物组。在GES-1细胞划痕损伤实验中,老香黄多糖各个组分均能加速损伤区域胃上皮细胞的生长,促进细胞向损伤区域迁移,恢复胃黏膜上皮细胞的完整性,阳性药物的划痕修复率最佳,其次是PFCP-2-1(50μg/mL)组分,两者无显著差异(P>0.05)。综上所述,老香黄多糖PFCP-2-1可能通过提高细胞抗氧化能力,以及促进GES-1细胞增殖、迁移发挥胃黏膜保护活性。In this study,Laoxianghuang polysaccharide was used as the experimental material to examine the protective effects on stomach mucosa.The polysaccharide was first extracted by hot water and then purified by Sevag methods deproteinization,DEAE-52 cellulose anion-exchange chromatography column,and CL-6B agarose gel column.The ethanol-damaged human gastric mucosal epithelial cells(GES-1)model and the GES-1 scratch injury model were used to evaluate gastric mucosal protective activity of the polysaccharide in vitro.Results showed that six fractions of Laoxianghuang polysaccharide were obtained,including PFCP,PFCP-1,PFCP-2,PFCP-3,PFCP-2-1,and PFCP-2-2.After ethanol damaged to GES-1,each fraction of Laoxianghuang polysaccharide improved cellular activity,increased the activity of superoxide dismutase(SOD),catalase(CAT),and inhibited malondialdehyde(MDA)content,exhibiting good antioxidant capacity to reduce mucosal damage.Among them,PFCP-2-1(50μg/mL)had the best effect on improving SOD enzyme activity and reducing MDA content,and PFCP-2-1(5μg/mL)had the best effect on improving CAT enzyme activity,which was significant compared with the model group(P<0.05)and better than the teprenone-positive drug group.It was demonstrated that Laoxianghuang polysaccharide all fractions accelerated gastric epithelial cell growth,promoted cell migration to the damaged area,and restored gastric mucosal epithelial cell integrity in the GES-1 scratch injury assay.Positive drugs had the most effective scratch repair rate,followed by PFCP-2-1(50μg/mL),but there was no significant difference between them(P>0.05).In conclusion,Laoxianghuang polysaccharide PFCP-2-1 may exert gastric mucosal protective activity by enhancing cellular antioxidant capacity as well as promoting the proliferation and migration of GES-1.

关 键 词：老香黄 多糖 GES-1 细胞 胃黏膜保护 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]