番茄红素对创伤性脑损伤小鼠炎性因子及肠黏膜屏障功能的影响  

Effect of lycopene on inflammatory factors and intestinal mucosal barrier function in mice with traumatic brain injury

作　　者：张洪玲 李才辉[1] 黄鸣恋 廖艳军 林天来[1] Zhang Hong-Ling;Li Cai-Hui;Huang Ming-Lian;Liao Yan-Jun;Lin Tian-Lai(Department of Critical Care Medicine,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou,Fujian 362000,China)

机构地区：[1]福建医科大学附属泉州第一医院重症医学科,福建泉州362000

出　　处：《解放军医学杂志》2023年第9期1011-1016,共6页Medical Journal of Chinese People's Liberation Army

基　　金：中华国际医学交流基金会黎介寿肠道屏障研究专项基金(LJS-2020C2)。

摘　　要：目的探讨番茄红素(Lyc)对创伤性脑损伤(TBI)小鼠炎性因子及肠黏膜屏障功能的影响。方法SPF级成年雄性BALB/c小鼠45只,采用随机数字表法分为假手术组、TBI组与TBI+Lyc组,每组15只。采用改良压缩损伤模型的方法制备TBI小鼠模型,假手术组不给予打击。各组按建模后1 d、2 d、3 d、7 d及14 d分为5个亚组,每亚组3只。假手术组及TBI组给予葵花油,TBI+Lyc组给予葵花油溶解的Lyc(浓度为1 mg/ml),剂量均为10 mg/(kg·d),每日固定时间灌胃,持续14 d。取各亚组小鼠下腔静脉血及回肠组织,采用ELISA法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、肠型脂肪酸结合蛋白(I-FABP)、二胺氧化酶(DAO)水平,比色法检测血清D-乳酸(D-LA)水平,终点显色法检测血清内毒素水平。Pearson相关检验分析炎性因子与肠黏膜屏障功能指标的相关性。HE染色观察回肠组织病理学变化,并测量回肠绒毛高度及隐窝深度。结果TBI组、TBI+Lyc组小鼠血清TNF-α、IL-1β、I-FABP、D-LA、DAO、内毒素水平随时间延长逐渐降低,但在各时间点均明显高于假手术组(P<0.01或P<0.001);TBI+Lyc组小鼠血清TNF-α、IL-1β、I-FABP、D-LA、DAO、内毒素水平在各时间点均明显低于TBI组(P<0.01或P<0.001)。血清TNF-α、IL-1β分别与I-FABP、D-LA、DAO、内毒素水平呈明显正相关关系(P<0.01)。假手术组回肠绒毛基本正常,TBI组回肠绒毛结构明显破坏,TBI+Lyc组回肠绒毛结构基本整齐。TBI组小鼠回肠绒毛高度和隐窝深度在各时间点均低于假手术组(P<0.05),TBI+Lyc组小鼠回肠绒毛高度和隐窝深度在各时间点均明显高于TBI组(P<0.05)。结论Lyc可降低TBI小鼠各个时期炎性因子水平,并可能通过抑制炎症反应来改善肠黏膜屏障功能。Objective To investigate the effect of lycopene(Lyc)on inflammatory factors and intestinal mucosal barrier function in mice with traumatic brain injury(TBI).Methods Forty-five SPF level of adult male BALB/c mice were randomly divided into sham group,TBI group and TBI+Lyc group,with 15 mice in each group.The TBI model was prepared by modified compression injury model,and the mice in sham group underwent an identical process without mechanical trauma.Each group was further divided into 5 subgroups at 1 d,2 d,3 d,7 d and 14 d after modelling(n=3/subgroup).Mice in sham group and TBI group were treated with 10 mg/kg sunflower oil,and in TBI+Lyc group were treated with 10 mg/kg sunflower oil-dissolved Lyc(concentration 1 mg/ml),daily gavage at a fixed time for 14 d.The inferior vena cava blood and ileum tissue of mice in each subgroup were collected.The serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),intestinal fatty acid binding protein(I-FABP)and diamine oxidase(DAO)were detected by ELISA,and the serum levels of D-lactic acid(D-LA)and endotoxin were detected by colorimetric method and endpoint chromogenic assay,respectively.Pearson correlation test was used to analyze the correlation between inflammatory factors and intestinal mucosal barrier function indicators.The pathological changes of ileum tissues were observed by HE staining,and the villus height and crypt depth of ileum were measured.Results The serum levels of TNF-α,IL-1β,I-FABP,D-LA,DAO and endotoxin of mice in TBI group and TBI+Lyc group gradually decreased with time,but were obviously higher than those in sham group at different time points(P<0.01 or P<0.001);the serum levels of TNF-α,IL-1β,I-FABP,D-LA,DAO and endotoxin of mice in TBI+Lyc group were significantly lower than those in TBI group at different time points(P<0.01 or P<0.001).Serum TNF-αand IL-1βwere significantly positively correlated with I-FABP,D-LA,DAO and endotoxin,respectively(P<0.01).The sham group showed basically normal ileal villi,the structure of ileal v

关 键 词：番茄红素 创伤性颅脑损伤 炎性因子 肠黏膜屏障功能 

分 类 号：R651.15[医药卫生—外科学]