基于常规Olsen法的全自动化学分析仪(SmartChem 200)测定土壤有效磷的研究  

Study on the method of determining soil available phosphorus (Olsen-P)by SmartChem 200 automatic chemical analyzer

作　　者：张向向 娄向弟 贺江 王永慧[1] ZHANG Xiang-xiang;LOU Xiang-di;HE Jiang;WANG Yong-hui(Jiangsu Coastal Area Institute of Agricultural Sciences,Yancheng Jiangsu 224000)

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224000

出　　处：《中国土壤与肥料》2023年第7期232-237,共6页Soil and Fertilizer Sciences in China

基　　金：江苏沿海地区农业科学研究所科研基金项目(YHS201908)。

摘　　要：土壤有效磷含量在评价土壤肥力、指导作物磷肥施用等方面有重要的意义。常规测定方法(碳酸氢钠浸提-人工钼锑抗比色法)步骤复杂、费时费力,提高常规方法测试效率和准确性是测土配方施肥研究中亟待解决的技术问题。选取4组不同有效磷含量的土壤为样品,探究基于碳酸氢钠浸提-全自动化学分析仪测定土壤有效磷含量的可行性。结果表明:此方法有效磷含量在0.00~2.00 mg/L范围内线性良好,相关系数r为1;用于测定土壤样品中有效磷含量,测定结果与常规方法接近,相对误差在0.00%~2.07%之间,有较高的相关性;用于测定土壤标准物质(NSA-2)中有效磷,测定结果的相对标准偏差(RSD,n=6)为0.37%,加标回收率为99.00%~101.00%。采用全自动化学分析仪测定土壤有效磷准确度高、精密度好,测定结果达到土壤有效磷的检测要求;同时该仪器操作简单、测试效率高,可用于大批量土壤有效磷含量的分析测试。The content of soil available phosphorus(AP)is one of the important factors to evaluate soil fertility and fertilization guidance.The normal operation(Olsen-P determined by artificial molybdenum-antimony colorimetric method combined with sodium bicarbonate extraction)is complicated,and takes more time.Improving the efficiency and accuracy of the conventional testing methods is an urgent technical problem to be solved in the soil testing and formula fertilization.This study was conducted to determine the AP contents in four groups of soil samples with different AP content using SmartChem 200 method.The results showed that the AP had good linear correlations in the range of 0.00~2.00 mg/L,and the correlation coefficient was 1.There were no significant difference between determination results of the SmartChem 200 method and those of the national standard method,and the relative errors were between 0.00%~2.07%.The AP content of national standard substance(NSA-2)results showed that the relative standard deviation was 0.37%,and the adding standard recoveries were between 99.00%~101.00%.The determination of soil AP by automatic chemical analyzer had high accuracy and precision,and the results could meet the requirements for soil AP testing.With the simple operation and high testing efficiency,the SmartChem 200 method can handle large numbers of soil samples.

关 键 词：土壤 有效磷 标准法 全自动化学分析仪 

分 类 号：S153.6[农业科学—土壤学]