SKH-1小鼠黑素瘤B16F10细胞种植瘤模型的建立  

Establishment of a SKH-1 mouse model of transplanted B16F10 melanoma

作　　者：张付贺 石磊[2] 罗敏[2] 刘沛[2] 范晴 王秀丽[2] Zhang Fuhe;Shi Lei;Luo Min;Liu Pei;Fan Qing;Wang Xiuli(Department of Dermatology,Shanghai Fengxian District Central Hospital,Shanghai 201499,China;Shanghai Skin Disease Hospital,Institute of Photomedicine,Tongji University School of Medicine,Shanghai 200443,China)

机构地区：[1]上海市奉贤区中心医院皮肤科,上海201499 [2]上海市皮肤病医院同济大学医学院光医学研究所,上海200443

出　　处：《中华皮肤科杂志》2023年第10期953-956,共4页Chinese Journal of Dermatology

基　　金：上海市奉贤区科技发展基金(20211820)。

摘　　要：目的建立SKH-1小鼠黑素瘤B16F10细胞皮下移植瘤和肺转移瘤模型。方法取SKH-1小鼠、C57BL/6小鼠各7只,于背部皮下接种5×106 B16F10细胞,观察并记录小鼠的生存时间,每3天用精密游标卡尺测量瘤体,计算肿瘤体积。肿瘤的长径>15 mm或出现恶病质或溃疡时判定为伦理学死亡。取5只SKH-1小鼠尾静脉注射5×106 B16F10细胞,观察小鼠的活动度、营养状态和生存时间,观察结束后处死小鼠,解剖并称量肺质量,对所有小鼠肺部行组织病理检查。实验重复3次。结果SKH-1小鼠在皮下接种后第6天接种部位皮肤开始出现黑点,渐发展为圆形黑色结节,直至肿瘤破溃、出血、死亡,伦理学死亡时间20~33 d。C57BL/6小鼠皮下接种第4天开始出现2~3 mm黑色小结节,发生伦理学死亡的时间为12~18 d。SKH-1小鼠第1~3次实验生存时间分别为(26.57±4.03)、(27.86±4.53)、(27.43±5.32)d,差异无统计学意(F=0.14,P=0.87);第27天时肿瘤体积分别为(1367.9±150.2)、(1452.0±50.1)、(1490.3±69.0)mm3,差异无统计学意义(F=0.92,P=0.46)。SKH-1小鼠尾静脉注射后第25天开始出现活动度减少、厌食等表现,第31天开始出现呆滞、消瘦、腹水、死亡等,伦理学死亡时间31~40 d;解剖后肺部见多处黑色结节,第1~3次实验小鼠生存时间分别为(34.2±2.58)、(36.4±2.60)、(34.8±2.38)d,差异无统计学意(F=1.01,P=0.39);小鼠肺部质量(156.1±18.5)、(164.0±19.6)、(172.0±17.2)mg,差异无统计学意(F=3.18,P=0.72)。所有小鼠全部成瘤,皮下肿物及肺部组织病理检查证实为黑素瘤。结论本实验成功建立SKH-1小鼠黑素瘤B16F10细胞皮下移植瘤和肺转移瘤模型,具有成瘤率高、成瘤稳定的特点。Objective To establish SKH-1 mouse models of subcutaneously transplanted B16F10 melanoma and of lung-metastasized B16F10 melanoma.Methods Seven SKH-1 mice and seven C57BL/6 mice were subcutaneously inoculated with 5×106 B16F10 cells on the back,and the survival duration of mice was observed and recorded.The tumor volume was measured by using a precision vernier caliper every 3 days.Mice were considered as ethically dead when the tumor length was more than 15 mm,or when cachexia or ulceration occurred.In addition,5 SKH-1 mice were injected with 5×106 B16F10 cells via the tail vein,and the activity,nutritional status and survival duration were observed.The mice were sacrificed after the observation,and the lungs were weighed after dissection.Histopathological examination was performed on the lungs of all mice.All the experiments were repeated 3 times.Results On day 6 after the subcutaneous inoculation,black spots appeared at the skin inoculation site in the SKH-1 mice,and gradually developed into round black nodules,then progressed into ulcerative and hemorrhagic tumors until the death of mice,and the time to ethical death ranged from 20 to 33 days.In the C57BL/6 mice,small black nodules measuring 2-3 mm in length appeared at the skin inoculation site on day 4 after the subcutaneous inoculation,and the time to ethical death ranged from 12 to 18 days.The survival duration of SKH-1 mice was 26.57±4.03,27.86±4.53,and 27.43±5.32 days in the 3 times of experiments respectively,and there was no significant difference(F=0.14,P=0.87);on day 27 after the subcutaneous inoculation,the tumor volume was 1367.9±150.2,1452.0±50.1,and 1490.3±69.0 mm3 in the 3 times of experiments respectively,and there was also no significant difference(F=0.92,P=0.46).SKH-1 mice had shown decreased activity and anorexia since day 25 after tail vein injections of B16F10 cells,and dullness,emaciation,ascites and death had been observed since day 31,and the time to ethical death ranged from 31 to 40 days;multiple black nodules were observed i

关 键 词：黑色素瘤 模型 动物 小鼠 无毛 小鼠 近交C57BL SKH-1小鼠 肿瘤模型 

分 类 号：R739.5[医药卫生—肿瘤] R-332[医药卫生—临床医学]