circCSNKIG1靶向miR-1180-5p对乳腺癌细胞生物学特性的影响  

Effect of circCSNKIG1 targeting miR-1180-5p on the biological characteristics of breast cancer cells

作　　者：孙小虎 刘燕[5] 曹旭晨[1,2,3,4] SUN Xiaohu;LIU Yan;CAO Xuchen(Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Tianjin 300060,China;Key Laboratory of Cancer Prevention and Therapy,Tianjin 300060,China;Tianjin's Clinical Research Center for Cancer,Tianjin 300060,China;Key Laboratory of Breast Cancer Prevention and Therapy,Tianjin Medical University,Ministry of Education,Tianjin 300060,China;Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,College of Pharmacy,Tianjin 300060,China)

机构地区：[1]天津医科大学肿瘤医院,国家恶性肿瘤临床医学研究中心,天津300060 [2]天津市肿瘤防治重点实验室,天津300060 [3]天津市恶性肿瘤临床医学研究中心,天津300060 [4]乳腺癌防治教育部重点实验室,天津300060 [5]天津市中医药研究院附属医院药学院,天津300131

出　　处：《现代肿瘤医学》2023年第19期3529-3534,共6页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:82274221);天津市医学重点学科(专科)建设项目(编号:TJYXZDXK-009A)。

摘　　要：目的:探讨circCSNKIG1靶向miR-1180-5p对乳腺癌细胞生物学特性的影响。方法:RT-qPCR分析circCSNKIG1和miR-1180-5p在乳腺癌组织、癌旁组织中的表达。分别转染si-circCSNKIG1、pcDNA-circCSNKIG1、miR-1180-5p模拟物、si-circCSNKIG1+anti-miR-1180-5p及相应的对照(si-NC、pcDNA、miR-NC和si-circCSNKIG1+anti-miR-NC)至MDA-MB-231细胞,利用划痕愈合、CCK-8、Transwell、集落形成实验检测细胞划痕愈合率、活力、侵袭数以及克隆数。荧光素酶报告基因法分析circCSNKIG1和miR-1180-5p靶向关系。结果:和癌旁组织相比,circCSNKIG1在乳腺癌组织中的表达高而miR-1180-5p表达低(P<0.05)。干扰circCSNKIG1表达显著降低细胞光密度(OD)值、侵袭数、克隆数以及划痕愈合率(P<0.05)。过表达miR-1180-5p显著降低细胞OD值、侵袭数、克隆数以及划痕愈合率(P<0.05)。circCSNKIG1靶向miR-1180-5p,且circCSNKIG1可以负调控miR-1180-5p表达。下调miR-1180-5p表达显著逆转干扰circCSNKIG1对MDA-MB-231细胞OD值、侵袭数、克隆数以及划痕愈合率的影响(P<0.05)。结论:干扰circCSNKIG1通过促进miR-1180-5p表达来抑制乳腺癌细胞增殖、迁移和侵袭能力。Objective:To explore the effect of circCSNKIG1 targeting miR-1180-5p on the biological characteristics of breast cancer cells.Methods:Expression of circCSNKIG1 and miR-1180-5p in breast cancer tissues and adjacent tissues was detected by RT-qPCR.si-circCSNKIG1,pcDNA-circCSNKIG1,miR-1180-5p mimic,si-circCSNKIG1+anti-miR-1180-5p and their corresponding negative controls(si-NC,pcDNA,miR-NC and si-circCSNKIG1+anti-miR-NC)were transfected into MDA-MB-231 cells respectively.And then the wound healing,CCK-8,Transwell,and colony formation assays were applied to detect the healing rate,viability,invasions number and clone number.Luciferase reporter gene method was used to determine the targeting relationship between circCSNKIG1 and miR-1180-5p.Results:Compared with adjacent normal tissues,circCSNKIG1 expression was higher and miR-1180-5p expression was lower in breast cancer tissues(P<0.05).Interference with the expression of circCSNKIG1 significantly reduced the cell optical density(OD)value,invasion numbers,clone numbers and scratch healing rate(P<0.05).Overexpression of miR-1180-5p significantly reduced the cell OD value,invasion numbers,clone numbers and scratch healing rate(P<0.05).circCSNKIG1 targeted miR-1180-5p,and circCSNKIG1 could negatively regulate miR-1180-5p expression.Downregulating of miR-1180-5p significantly reversed the effect of interfering with circCSNKIG1 on the OD value,invasion numbers,clone numbers and scratch healing rate(P<0.05).Conclusion:Interference with circCSNKIG1 inhibits breast cancer cell proliferation,migration and invasion via increasing miR-1180-5p expression.

关 键 词：乳腺癌 circCSNKIG1 miR-1180-5p 增殖 迁移 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]