miR-506-3p通过靶向结合SART3抑制葡萄膜黑色素瘤恶性表型  

Mechanism of miR-506-3p inhibiting malignant phenotypes of uveal melanoma by targeting SART3

作　　者：陈瑜 张永珍 王永强 王宗仁[1] 胡莹莹[2] CHEN Yu;ZHANG Yongzhen;WANG Yongqiang;WANG Zongren;HU Yingying(Department of Laboratory Medicine,Xinxiang Central Hospital,Henan Xinxiang 453000,China;Department of Emergency,the First Affiliated Hospital of Henan University of Science and Technology,Henan Luoyang 471000,China)

机构地区：[1]新乡市中心医院检验科,河南新乡453000 [2]河南科技大学第一附属医院急诊科,河南洛阳471000

出　　处：《现代肿瘤医学》2023年第19期3557-3565,共9页Journal of Modern Oncology

基　　金：河南省医学科技攻关计划项目(编号:2018020283)。

摘　　要：目的:探讨miR-506-3p通过靶向结合鳞状细胞癌抗原3(squamous cell carcinoma antigen 3,SART3)抑制葡萄膜黑色素瘤(uveal melanoma,UM)恶性表型的机制。方法:实验将OCM-1A和MUM-213细胞构建miR-506-3p过表达模型(miR-NC组和miR-506-3p组),构建SART3敲低模型(shNC组、shSART31#组和shSART32#组)。生物信息学分析miR-506-3p和SART3与UM不良表型之间的关系并预测两者之间的靶向关系。RT-PCR检测miR-506-3p和SART3在APRE-19、OCM-1A和MUM-213细胞中的表达。CCK-8实验、细胞划痕愈合实验和侵袭实验分别检测细胞增殖、横向迁移和侵袭能力。裸鼠成瘤实验检测瘤体重量、体积。Western Blot实验检测SART3蛋白表达。双荧光酶素实验验证miR-506-3p和SART3的靶标关系。结果:miR-506-3p在UM细胞中低表达,过表达miR-506-3p能抑制细胞增殖、迁移和侵袭能力。上调miR-506-3p表达可以抑制裸鼠移植瘤生长。数据库预测SART3是miR-506-3p的靶基因,双荧光酶素报告实验证实SART3是miR-506-3p作用靶点。SART3在UM中高表达,下调SART3表达可以抑制UM细胞增殖、迁移和侵袭。miR-506-3p靶向结合SART3抑制UM细胞增殖、迁移和侵袭。结论:miR-506-3p能抑制UM细胞增殖、迁移和侵袭,其作用机制可能是通过下调SART3表达实现的。Objective:To explore the mechanism of miR-506-3p inhibiting uveal melanoma(UM)by targeting squamous cell carcinoma antigen 3(SART3).Methods:In this experiment,OCM-1A and MUM-213 cells were applied to construct miR-506-3p overexpression models(miR-NC group,miR-506-3p group)and SART3 knocking-down models(shNC group,shSART31#group,shSART32#group).The relationship between miR-506-3p,SART3 and poor phenotypes of UM was analyzed by bioinformatics,and the targeted relationship between the two indexes was predicted.The expressions of miR-506-3p and SART3 in APRE-19,OCM-1A and MUM-213 cells were detected by RT-PCR.CCK-8 test,scratch healing test and invasion test were used to detect the ability of cell proliferation,lateral migration and invasion.The weight and volume of tumors were detected by tumor formation assay in nude mice.The expression of SART3 protein was detected by Western Blot.The targeted relationship between miR-506-3p and SART3 was verified by dual luciferin assay.Results:The expression of miR-506-3p was down-regulated in UM cells,and overexpression of miR-506-3p could inhibit cells proliferation,migration and invasion.Up-regulating the expression of miR-506-3p could inhibit the growth of transplanted tumors in nude mice.It was predicted by database that SART3 was the target gene of miR-506-3p,which was verified by dual luciferin assay.The expression of SART3 was up-regulated in UM.Down-regulating the expression of SART3 could inhibit the proliferation,migration and invasion of UM cells.The miR-506-3p could inhibit the proliferation,migration and invasion of UM cells by targeting SART3.Conclusion:The miR-506-3p can inhibit the proliferation,migration and invasion of UM cells,and its mechanism of action may be related to down-regulating SART3 expression.

关 键 词：miR-506-3p 葡萄膜黑色素瘤 增殖 鳞状细胞癌抗原3 

分 类 号：R739.5[医药卫生—肿瘤]