人参皂苷Rg3对帕金森病模型大鼠多巴胺能神经元氧化应激的抑制作用  

Inhibitory Effect of Ginsenoside Rg3 on Oxidative Stress in Dopaminergic Neurons of Parkinson's Disease Model Rats

作　　者：侯晓丽 张亚慧 唐迎乐 HOU Xiao-li;ZHANG Ya-hui;TANG Ying-le(Medical School of Yangzhou Polytechnic College,Yangzhou 225000,Jiangsu,China;Yangzhou University Medical College,Yangzhou 225000,Jiangsu,China)

机构地区：[1]扬州市职业大学医学院,江苏扬州225000 [2]扬州大学医学院,江苏扬州225000

出　　处：《医学信息》2023年第19期88-91,共4页Journal of Medical Information

基　　金：江苏省大学生科创基金资助项目(编号:202211117079Y、202111117069Y)。

摘　　要：目的研究人参皂苷Rg3对帕金森病模型大鼠黑质多巴胺能神经元氧化应激的抑制作用,探讨Rg3对多巴胺能神经元保护作用的机制。方法将24只SD成年雄性大鼠随机分为对照组、PD模型组、Rg3组及阳性药物组,每组6只,分别编号1~6号;造模前3 d开始给药,Rg3组给予6 mg/kg Rg3灌胃,阳性药物组给予11 mg/kg司来吉兰灌胃,对照组及模型组给予6 mg/kg羧甲基纤维素钠灌胃,1次/d,连续31 d;灌胃3 d后,除对照组外的其余3组大鼠均皮下注射鱼藤酮纳米脂质载体(R-NLC),首剂量给予0.5 mg/kg,第2次给予0.8 mg/kg,此后每次1 mg/kg,2 d/次,连续28 d,对照组皮下注射同等剂量空白纳米脂质载体。采用外观行为表现评分考察大鼠外观行为学表现,采用黄嘌呤氧化酶法和二硫代二硝基苯甲酸法测定组织匀浆中SOD、GSH-PX活力,采用硫代巴比妥缩合法测定MDA含量。结果各组大鼠不同时间点行为学评分比较,差异有统计学意义(P<0.05);与对照组相比,模型组大鼠黑质MDA含量升高、黑质SOD活性及黑质GSH-PX活性降低,差异有统计学意义(P<0.05);与模型组相比,Rg3组及阳性药物组黑质MDA含量降低,黑质SOD活性及黑质GSH-PX活性升高,差异有统计学意义(P<0.05)。结论Rg3具有抗氧化应激作用,可通过抗氧化应激实现对神经细胞的保护作用。Objective To study the inhibitory effect of ginsenoside Rg3 on oxidative stress in dopaminergic neurons of Parkinson's disease model rats,and to explore the mechanism of protective effect of Rg3 on dopaminergic neurons.Methods A total of 24 adult male SD rats were randomly divided into control group,PD model group,Rg3 group and positive drug group,with 6 rats in each group,numbered 1-6,respectively.Three days before modeling,Rg3 group was given 6 mg/kg Rg3 by gavage,positive drug group was given 11 mg/kg selegiline by gavage,control group and model group were given 6 mg/kg sodium carboxymethyl cellulose by gavage,once a day for 31 consecutive days.After 3 days of gavage,rats in the other 3 groups except the control group were injected subcutaneously with rotenone nano lipid carrier(R-NLC),0.5 mg/kg at the first dose,0.8 mg/kg at the second dose,and then 1 mg/kg each time,once every 2 days,for consecutive 28 days.The control group was injected subcutaneously with the same dose of blank nano lipid carrier.Appearance and behavior scores were used to investigate the appearance and behavior of rats.Activities of SOD and GSH-PX in tissue homogenate were determined by xanthine oxidase method and dithiobarbitone method,and MDA content was determined by thiobarbitone reduction method.Results There were significant differences in the behavioral scores of rats in each group at different time points(P<0.05).Compared with the control group,the content of MDA in the substantia nigra of the model group increased,the activity of SOD in the substantia nigra and the activity of GSH-PX in the substantia nigra decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the content of MDA in the substantia nigra of the Rg3 group and the positive drug group decreased,the activity of SOD in the substantia nigra and the activity of GSH-PX in the substantia nigra increased,and the difference was statistically significant(P<0.05).Conclusion Rg3 has obvious anti-oxidative stress effect and can protect n

关 键 词：帕金森病 人参皂苷RG3 氧化应激 神经保护 鱼藤酮 

分 类 号：R742.5[医药卫生—神经病学与精神病学]