大叶茜草素对人肝癌细胞生物学行为的影响及作用机制  

Effect and mechanism of mollugin on biological behavior of human hepatocellular carcinoma cells

作　　者：陈博婷 郭小艳 CHEN Boting;GUO Xiaoyan(Department of Geriatric Diseases,Xi'an No.9 Hospital,Xi'an 710054,Shaanxi Province,China)

机构地区：[1]西安市第九医院老年病一科,陕西西安710054

出　　处：《世界临床药物》2023年第8期783-790,864,共9页World Clinical Drug

基　　金：西安市第九医院科研项目(2022yb16)。

摘　　要：目的探讨大叶茜草素对人肝癌细胞生物学行为的影响及作用机制。方法将HepG2细胞分为对照组、大叶茜草素低、中、高浓度(40、60及80 mmol/L)组、SN50(36μmol/L)组、大叶茜草素高浓度(80 mmol/L)+LPS(2.5μmol/L)组。采用细胞计数-8法、克隆形成实验、流式细胞术以及Transwell染色法检测HepG2细胞的增殖、凋亡及侵袭迁移情况;蛋白质印迹法检测HepG2细胞中蛋白表达情况。结果与对照组相比,大叶茜草素低、中、高浓度组及SN50组的细胞增殖抑制率、凋亡率、兔抗半胱天冬酶-3(Caspase-3)、B淋巴细胞瘤2相关X蛋白(B-cell lymphoma 2 associated x protein,Bax)、核因子κB抑制蛋白-α(inhibition of nuclear factor-κB,IκB-α)以及Bax/B淋巴细胞瘤2(B-cell lymphoma 2,Bcl-2)均升高(P<0.05);克隆细胞数、侵袭和迁移细胞数、Bcl-2、基质金属蛋白酶(ma-trix metalloproteinase,MMP)-2、MMP-9、核因子(nuclear factor,NF)-κB以及p-IκB-α均降低(P<0.05);与大叶茜草素高浓度组相比,大叶茜草素高浓度+LPS组细胞增殖抑制率、凋亡率、Caspase-3、Bax、IκB-α以及Bax/Bcl-2均降低,克隆细胞数、侵袭和迁移细胞数、MMP-2、MMP-9、NF-κB、Bcl-2以及p-IκB-α均升高(P<0.05)。结论大叶茜草素可能通过抑制NF-κB通路,抑制HepG2细胞增殖、侵袭以及迁移,并促进细胞凋亡,有望成为治疗肝癌的靶向药物。Objective To investigate the effect of mollugin on the biological behavior of human hepatocellular carcinoma cells and its mechanism.Methods HepG2 cells were divided into control group,low,medium and high concentration of mollugin(40,60 and 80 mmol/L)group,SN50(36μmol/L)group and high concentration of mollugin(80 mmol/L)+LPS(2.5μmol/L)group.Cell counting kit-8 assay,clonal formation assay,flow cytometry and Transwell staining were used to detect the proliferation,apoptosis,invasion and migration of HepG2 cells.Western blotting was used to detect the protein expression in HepG2 cells.Results Compared with the control group,the cell proliferation inhibition rate,apoptosis rate,rabbit anti-Caspase-3(Caspase-3),B-cell lymphoma 2 associated X protein(Bax),inhibitor of nuclear factor-κB(IκB-α)and Bax/B-cell lymphoma 2(Bcl-2)in the low,medium,and high concentration of mollugin groups and SN50 group were increased(P<0.05).The number of clone cells,invasion and migration cells,Bcl-2,matrix metalloproteinase(MMP)-2,MMP-9,nuclear factor(NF)-κB and p-IκB-αwere all decreased(P<0.05).Compared with the high concentration of mollugin group,the cell proliferation inhibition rate,apoptosis rate,Caspase-3,Bax,IκB-αand Bax/Bcl-2 in high concentration of mollugin+LPS group were decreased,the number of cloned cells,invasive and migratory cells,and the expression of MMP-2,MMP-9,NF-κB,Bcl-2 and p-IκB-αwere all increased(P<0.05).Conclusion Mollugin may inhibit cell proliferation,invasion,migration and promote apoptosis of HepG2 cells by inhibiting NF-κB pathway,which is expected to become a targeted drug for the treatment of liver cancer.

关 键 词：大叶茜草素 HEPG2 增殖 凋亡 迁移 侵袭 

分 类 号：R-33[医药卫生]