非肌层浸润膀胱癌组织中核糖核酸结合蛋白15及泛素特异性肽酶24表达的临床预后意义  

Clinical prognostic significance of expression of ribonucleic acid binding protein 15 and ubiquitin specific peptidase 24 in non muscle invasive bladder cancer

作　　者：何丹 石艳宏 王峥[2] 张晓炜[2] 王冠杰[2] He Dan;Shi Yanhong;Wang Zheng;Zhang Xiaowei;Wang Guanjie(Department of Pathology,Xi'an Central Hospital,Shaanxi Province,Xi'an 712000,China;不详)

机构地区：[1]西安市中心医院病理科,710043 [2]西安市中心医院肿瘤科,710043 [3]咸阳市中心医院病理科,712000

出　　处：《疑难病杂志》2023年第10期1027-1031,1038,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省卫生健康科研基金项目(2019D0104)。

摘　　要：目的研究非肌层浸润膀胱癌(NMIBC)组织中核糖核酸结合蛋白15(RBM15)、泛素特异性肽酶24(USP24)蛋白表达及与临床预后的关系。方法选取2018年1月—2020年1月西安市中心医院肿瘤科诊治NMIBC患者90例为研究对象。应用免疫组化法检测癌及癌旁组织中RBM15、USP24蛋白表达;比较不同临床病理特征NMIBC患者RBM15、USP24蛋白表达差异;Spearman秩相关分析膀胱癌组织中RBM15与USP24蛋白表达的相关性。随访3年,应用Kaplan-Meier曲线分析(Log-Rank检验)RBM15、USP24蛋白表达对无进展生存预后的影响;多因素COX模型分析NMIBC患者无进展生存预后的影响因素。结果膀胱癌组织中RBM15、USP24蛋白阳性率分别为66.67%(60/90)、74.44%(67/90),高于癌旁组织6.67%(6/90)、11.11%(10/90),差异有统计学意义(χ^(2)=69.761、73.739,P均<0.001)。NMIBC癌组织中RBM15与USP24表达呈正相关(r=0.716,P<0.001)。NMIBC癌组织中RBM15、USP24阳性率在肿瘤分期T1期、病理分级高级别中分别高于肿瘤分期Ta/Tis期、病理分级低级(χ^(2)/P=11.903/0.001,10.866/0.001;17.457/<0.001,11.433/0.001)。RBM15阳性组和阴性组3年无进展生存率分别为45.00%(27/60)和90.00%(27/30)。USP24阳性组和阴性组3年无进展生存率分别为50.75%(34/67)和86.96%(20/23)。RBM15阳性组、USP24阳性组累积无进展生存率明显低于RBM15阴性组、USP24阴性组(χ^(2)/P=8.057/0.005、15.379/<0.001)。COX模型分析结果表明,肿瘤分期T1期、病理分级高级别、RBM15阳性、USP24阳性是影响NMIBC患者无进展生存期的独立危险因素[OR(95%CI)=1.614(1.227~2.214),1.917(1.319~2.799),1.839(1.228~2.753),1.744(1.245~2.443)]。结论NMIBC癌组织中RBM15、USP24表达均升高,两者与肿瘤分期及病理分级有关,是影响NMIBC患者无进展生存期的独立危险因素。Objective To study the expression of ribonucleic acid binding protein 15(RBM15)and ubiquitin specific peptidase 24(USP24)in non muscle invasive bladder cancer(NMIBC)and their relationship with clinical prognosis.Methods Ninety patients with NMIBC diagnosed and treated in the Oncology Department of Xi'an Central Hospital from January 2018 to January 2020 were selected as the research subjects.Immunohistochemical method was used to detect the expression of RBM15 and USP24 proteins in cancer and adjacent tissues.Compare the differences in protein expression of RBM15 and USP24 in NMIBC patients with different clinical and pathological characteristics.Spearman rank correlation analysis was used to analyze the correlation between RBM15 and USP24 protein expression in bladder cancer tissues.Follow up for 3 years and use Kaplan Meier curve analysis(Log Rank test)to investigate the impact of RBM15 and USP24 protein expression on the prognosis of progression free survival.Multivariate COX model analysis of the influencing factors of progression free survival prognosis in NMIBC patients.Results The positive rates of RBM15 and USP24 protein in bladder cancer tissues were 66.67%(60/90)and 74.44%(67/90),respectively,higher than that in adjacent tissues 6.67%(6/90)and 11.11%(10/90),with a statistically significant difference(χ2=69.761,73.739,both P<0.001).There is a positive correlation between the expression of RBM15 and USP24 in NMIBC cancer tissue(r=0.716,P<0.001).The positive rates of RBM15 and USP24 in NMIBC cancer tissue were higher in tumor stage T1 and higher in pathological grading than in tumor stage Ta/tis and lower in pathological grading,respectively(χ2/P=11.903/0.001,10.866/0.001;17.457/<0.001,11.433/0.001).The 3-year progression free survival rates of the RBM15 positive group and the negative group were 45.00%(27/60)and 90.00%(27/30),respectively.The 3-year progression free survival rates of the USP24 positive and negative groups were 50.75%(34/67)and 86.96%(20/23),respectively.The cumulative progression free s

关 键 词：膀胱癌 非肌层浸润 核糖核酸结合蛋白15 泛素特异性肽酶24 无进展生存 预后 

分 类 号：R737.14[医药卫生—肿瘤]