LncRNA CUST_49525和CUST_47163在急性髓系白血病患者外周血中的表达及其诊断意义  

Expression and diagnostic significance of LncRNA CUST_49525 and CUST_47163 in peripheral blood of patients with acute myeloid leukemia

作　　者：高冉 哈丽亚·塔力达吾别克 刘晓东 田薇[1] GAO Ran;HALIYA Talidawubieke;LIU Xiaodong;TIAN Wei(School of Public Health,Xinjiang Medical University,Urumqi 830011,Xinjiang,China)

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830011

出　　处：《癌变．畸变．突变》2023年第5期355-359,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆维吾尔自治区高校科研计划(XJEDU2020Y023)。

摘　　要：目的:探讨外周血淋巴细胞中LncRNA CUST_49525和CUST_47163在急性髓系白血病(AML)中的表达及其诊断价值。方法:采用实时荧光定量PCR(qPCR)检测AML患者与健康对照组人群外周血淋巴细胞中LncRNA CUST_49525和CUST_47163的表达水平,并通过胞质分裂阻滞微核试验检测两组人群淋巴细胞微核率。利用非参数Mann-Whitney U检验分析其差异,并绘制ROC曲线分析其诊断意义。结果:与对照组比较,AML患者外周血淋巴细胞中LncRNA CUST_49525和CUST_47163的表达水平均下调(P<0.01),而淋巴细胞微核率显著升高(P<0.01)。ROC曲线分析结果显示,LncRNA CUST_49525在外周血淋巴细胞中表达的ROC曲线下面积为0.742,其95%CI为0.627~0.858;LncRNA CUST_47163的ROC曲线下面积为0.859,其95%CI为0.769~0.949。外周血淋巴细胞微核细胞率的ROC曲线下面积为0.948,其95%CI为0.899~0.997。三者联合诊断的ROC曲线下面积为0.970,95%CI为0.931~1.000。与对角线下面积(0.500)相比,CUST_49525、CUST_47163、微核率和联合诊断各组AUC均增大,差异具有统计学意义(P<0.01),且联合诊断效果优于单一指标诊断,单一指标诊断中微核率优于其他指标(P<0.01)。结论:AML患者外周血淋巴细胞中LncRNA CUST_49525和CUST_47163表达水平均下调,二者与淋巴细胞微核率的联合诊断效果优于单一指标,可作为AML诊断的潜在参考指标。OBJECTIVE:To investigate expression and diagnostic values of LncRNA CUST_49525 and CUST_47163 in peripheral blood lymphocytes in patients with acute myeloid leukemia(AML).METHODS:Real time fluorescence quantitative PCR(qPCR)was used to detect expression levels of LncRNA CUST_49525 and CUST_47163 in peripheral blood lymphocytes of patients and healthy controls.The cytokinesis-arrest micronucleus test was used to detect micronuclei levels in lymphocytes.The collected data were evaluated using the non-parametric Mann Whitney U test to determine significant differences and ROC curves were drawn to determine diagnostic significance.RESULTS:Compared with the control group,the expression levels of both LncRNA CUST_49525 and CUST_47163 from the patients were significantly down-regulated(P<0.01),while the micronucleus rates were significantly increased(P<0.01).The ROC curve analyses show that the area under the ROC curve of LncRNA CUST_49525 in the patients was 0.742,with a 95%CI of 0.627-0.858;and that for LncRNA CUST_47163 was 0.859,with a 95%CI of 0.769-0.949.The area under the ROC curve for the micronucleus rates was 0.948,with a 95%CI of 0.899-0.997(P<0.01).The area under the ROC curve for the combined diagnosis of the three was 0.970,with a 95%CI of 0.931-1.000(P<0.01).CONCLUSION:The expression levels of LncRNA CUST_49525 and CUST_47163 in peripheral blood lymphocytes of AML patients were abnormally down-regulated.The combined diagnostic effect of the two together with the micronucleus rate was superior to a single indicator,and can be a potential reference indicator for AML diagnosis.

关 键 词：急性髓系白血病 长链非编码RNA 微核 诊断 

分 类 号：R733.71[医药卫生—肿瘤]