重组人生长激素对棕榈酸诱导的HepG2细胞脂质沉积的影响  

Effects of recombinant human growth hormone on palmitic acid induced lipid deposition in HepG2 cells

作　　者：张芳 冉丽媛 张金金 吴英杰 ZHANG Fang;RAN Liyuan;ZHANG Jinjin;WU Yingjie(School of Laboratory Animals,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;Shandong Provincial Hospital,Jinan 250012,China;Science and Technology Innovation Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)实验动物学院,山东济南250117 [2]山东第一医科大学附属省立医院模式动物研究所,山东济南250012 [3]山东第一医科大学(山东省医学科学院)医学科技创新中心,山东济南250117

出　　处：《山东第一医科大学（山东省医学科学院）学报》2023年第9期641-646,共6页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：国家重点研发计划(2021YFF0702102)。

摘　　要：目的探讨重组人生长激素(recombinant human growth hormone,rhGH)对体外诱导的脂肪肝细胞模型脂质代谢的影响,为进一步拓广rhGH在临床中的应用提供理论支撑。方法利用棕榈酸(palmitic acid,PA)诱导HepG2细胞脂质积累,构建脂肪肝细胞模型,对细胞模型给予不同浓度的rhGH处理,利用CCK8实验检测不同浓度PA和rhGH对HepG2细胞存活率的影响,通过油红O染色评估HepG2细胞中的脂质积累水平,采用实时荧光定量PCR(real‐time fluorescence quantitative PCR,RT‐qPCR)和Western blot评估HepG2细胞系内脂质代谢相关基因和蛋白表达水平。结果利用0.2 mmol/L PA,成功诱导了脂肪肝细胞模型,该模型表现为细胞质内脂滴显著积聚。经rhGH刺激后,胞内脂滴积聚随rhGH浓度升高而降低,40μg/L的rhGH给药浓度可达到显著降脂效果。RT‐qPCR和Western blot结果显示,rhGH处理后细胞内脂质合成相关基因和蛋白表达显著下调,脂质分解相关基因和蛋白表达无明显变化。结论rhGH可通过抑制脂肪酸从头合成途径来减轻PA诱导的HepG2细胞中的脂质沉积。Objective:To explore the effect of recombinant human growth hormone(rhGH)on the lipid metabolism of fatty liver cell model induced in vitro,and to provide theoretical support for further expanding the application of rhGH in clinical practice.Methods:Palmitic acid(PA)was combined to induce lipid accumulation in HepG2 cells,and a fatty liver cell model was constructed.The cell model was treated with different concentrations of rhGH,and CCK8 assay was used to detect the effects of different concentrations of PA and rhGH on the survival of HepG2 cells.The lipid accumulation level in HepG2 cells was evaluated by oil red O staining,the expression levels of genes and proteins related to lipid metabolism in HepG2 cell lines were evaluated by RT-qPCR and Western blot,and the mechanism of action of rhGH was preliminarily explored.Results:In this study,0.2 mmol/L PA successfully induced a fatty liver cell model,which showed significant accumulation of lipid droplets in the cytoplasm.After being stimulated by rhGH,the accumulation of intracellular lipid droplets decreased with the increase of rhGH concentration,and the rhGH concentration of 40 ng/mL achieved a significant lipid-lowering effect.The results of RT-qPCR and Western blot showed that the expression of genes and proteins related to lipid synthesis in cells were significantly down-regulated after rhGH treatment,while the expressions of genes and proteins related to lipolysis had no significant changes.Conclusion:rhGH can alleviate the lipid deposition in HepG2 cells induced by PA by inhibiting the de novo fatty acid synthesis pathway.

关 键 词：重组人生长激素 脂肪肝细胞模型 棕榈酸 脂质代谢 

分 类 号：R575.5[医药卫生—消化系统]