南海冷泉区深海沉积物来源放线菌的分离培养及其抑菌活性  

Isolation,cultivation and antibacterial activity of actinomycetes from the sediments in the deep-sea cold seeps of South China Sea

作　　者：陈以秋 韩梅桂 韩壮 唐敏[1] CHEN Yiqiu;HAN Meigui;HAN Zhuang;TANG Min(School of Ecological and Environmental Sciences,Hainan University,Haikou,Hainan 570228,China;Sanya Institute of Deep-sea Science and Engineering,Chinese Academy of Sciences,Sanya,Hainan 572000,China)

机构地区：[1]海南大学生态与环境学院,海口570228 [2]中国科学院深海科学与工程研究所,三亚572000

出　　处：《热带生物学报》2023年第5期545-551,共7页Journal of Tropical Biology

基　　金：国家自然科学基金项目(32160270,31660128);海南省重大科技计划项目(ZDKJ2019011)。

摘　　要：为挖掘南海冷泉区可培养放线菌资源及其潜在的生物活性,采用稀释涂布法分离培养了来源于南海冷泉区深海沉积物中的放线菌,利用16S rRNA基因测序鉴定菌株,构建系统发育树,并分析了放线菌的多样性;通过K-B法药敏试验对放线菌发酵粗提物进行抑菌活性测试。结果显示:分离获得的50株放线菌隶属于5个科,7个属,其中5株放线菌被初步判定为潜在新种。7株放线菌的发酵粗提物对副溶血性弧菌、甲氧西林敏感金黄色葡萄球菌和甲氧西林耐药金黄色葡萄球菌呈现不同程度的抑菌活性,其中活性最好的为小单孢菌属(Micromonospora)的2株菌,Micromonospora sp.LDBS3988抑菌活性最强,Micromonospora sp.LDBS3949抑菌活性较为广谱。In order to explore the resources and potential bioactivity value of actinomycetes,actinomycetes were isolated from the deep-sea sediments in the cold seeps of South China Sea by serial dilution and spread plate technique and cultured.The strains of isolated actinomycetes were identified by using 16S rRNA gene sequencing,based on which a phylogenetic tree was constructed,and the diversity of actinomycetes were analyzed.The antibacterial activities of the crude extracts of the isolated actinomycetes were tested by using the K-B test for antibiotic susceptibility.The results showed that 50 isolated actinomyces belonged to 5 families and 7 genera,and that 5 strains of actinomycetes were preliminarily identified as potential new species.And the crude extracts of 7 strains showed different antibacterial activities against methicillin-resistant Staphylococcus aureus,methicillin-sensitive Staphylococcus aureus and Vibrio parahaemolyticus.Among the 7 strains,two strains of Micromonospora displayed the highest antibacterial activity.Micromonospora sp.LDBS3988 has the highest antibacterial activity,and Micromonospora sp.LDBS3949 has a broad-spectrum antibacterial activity.

关 键 词：深海沉积物 放线菌 抑菌活性 冷泉 

分 类 号：Q93-331[生物学—微生物学]