泛癌组织中T-box转录因子19基因水平与患者预后和肿瘤免疫微环境的相关性  

Correlations of TBX19 gene in pan-cancer tissues with prognosis and tumor immune microenvironment

作　　者：何梦婷 刘宏劲 任宏生 HE Mengting;LIU Hongjin;REN Hongsheng(The Second Clinical Medical School,Shandong University of Traditional Chinese Medicine,Jinan 250001,China)

机构地区：[1]山东中医药大学第二临床医学院,济南250001 [2]山东第一医科大学附属省立医院东院重症医学科

出　　处：《山东医药》2023年第27期47-53,共7页Shandong Medical Journal

基　　金：齐鲁卫生与健康领军人才资助项目(2021.01.01-2025.12.31)。

摘　　要：目的基于数据库数据分析泛癌组织中T-box转录因子19(TBX19)表达水平与患者预后和肿瘤免疫微环境(免疫细胞浸润、免疫检查点基因、肿瘤突变负荷和微卫星不稳定性)之间的关系。方法从癌症基因组图谱(TCGA)数据库中获取泛癌组织及相应正常组织中TBX19表达水平,比较不同WHO分级和TNM分期泛癌组织中TBX19表达水平,使用Cox回归分析TBX19表达水平与肿瘤患者预后的关系。基于肿瘤免疫估算数据库(TIMER2)数据,采用Pearson法分析TBX19表达水平与免疫细胞浸润丰度的相关性;在Sanger Box网站中利用TCGA数据库采用Pearson方法分析TBX19表达水平与免疫评分、肿瘤突变负荷和微卫星不稳定性的相关性;基于临床生信之家数据库数据,采用Spearman法分析TBX19表达水平与免疫检查点基因的相关性。结果在膀胱尿路上皮癌、胆管癌、结肠癌(COAD)、结直肠癌(COADREAD)、食管癌、头颈鳞状细胞癌、肾透明细胞癌、肾乳头状细胞癌、混合肾癌、肝细胞癌(LIHC)、肺腺癌、肺鳞癌、嗜铬细胞瘤和副神经节瘤、直肠腺癌、胃癌、胃和食管癌癌组织中TBX19高表达(P均<0.05),在乳腺浸润癌、肾嫌色细胞癌、前列腺癌、子宫内膜癌癌组织中TBX19低表达(P均<0.05)。不同WHO分级以及TNM分期的COAD、COADREAD癌组织中TBX19表达差异有统计学意义(P均<0.05)。与TBX19低表达者比较,LIHC癌组织TBX19高表达者的总体生存期、疾病特异性生存期、无疾病间隔期和无进展间隔期短(P均<0.05)。在COAD、KIRC、LIHC等多种癌组织中,TBX19的表达与免疫细胞浸润、免疫检查点基因、肿瘤突变负荷、微卫星不稳定性密切相关(P均<0.05)。结论泛癌组织中TBX19表达水平以升高为主,TBX19高表达者预后差;癌组织中TBX19高表达与肿瘤免疫微环境有关,在多种肿瘤中免疫浸润水平升高、肿瘤突变负荷增大、微卫星不稳定性增加。Objective To analyze the relationships of T-box transcription factor 19(TBX19)expression levels in pan-cancer tissues with patients'prognosis and the tumor immune microenvironment(immune cell infiltration,immune checkpoint genes,tumor mutation burden,and microsatellite instability)based on database data.Methods TBX19 ex⁃pression levels in the pan-cancer tissues and corresponding normal tissues were obtained from The Cancer Genome Atlas(TCGA)database,and the TBX19 expression levels were compared in the pan-cancer tissues of patients with different WHO grades and TNM stages.Using Cox regression to analyze the relationship between TBX19 expression levels and the prognosis of tumor patients.The correlation between TBX19 expression levels and the abundance of immune cell infiltra⁃tion was analyzed by Pearson method based on data from the Tumor Immune Estimation Resource(TIMER2).Correlations of TBX19 expression levels with immune scores,tumor mutation burden and microsatellite instability were analyzed by Pearson method using TCGA database in the Sanger Box website.Based on the Clinical Biosense House database data,the correlations between TBX19 expression levels and immune checkpoint genes were analyzed by the Spearman method.Re⁃sults TBX19 was highly expressed in bladder urothelial carcinoma,cholangiocarcinoma,colon adenocarcinoma(CO⁃AD),colorectal carcinoma(COADREAD),esophageal carcinoma,head and neck squamous cell carcinoma,renal clear cell carcinoma,renal papillary cell carcinoma,pan-kidney cohort,hepatocellular carcinoma(LIHC),lung adenocarcino⁃ma,lung squamous cell carcinoma,pheochromocytoma and paraganglioma,rectum adenocarcinoma,gastric carcinoma,and stomach and esophageal carcinoma tissues(all P<0.05),and was low expressed in breast invasive carcinoma,kidney chromophobe cell tumor,prostate adenocarcinoma,and endometrial carcinoma tissues(all P<0.05).There were statisti⁃cally significant differences in the TBX19 expression in COAD and COADREAD cancer tissues with different WHO grades as well as dif

关 键 词：T盒转录因子19 泛癌分析 肿瘤免疫微环境 免疫浸润细胞 肿瘤突变负荷 微卫星不稳定性 

分 类 号：R730.2[医药卫生—肿瘤]