circDOCK1靶向miR-3194-3p调控人肺癌细胞增殖、迁移和侵袭  

circDOCK1 Targets miR-3194-3p to Regulate the Proliferation,Migration and Invasion of Human Lung Cancer Cells

作　　者：甄伟 肖琳 宣立功[3] 张静[2] 刘镓齐 ZHEN Wei;XIAO Lin;XUAN Ligong;ZHANG Jing;LIU Jiaqi(Department of Traditional Chinese Medicine,Tangshan People’s Hospital,Tangshan 063001,China;Department of Integrated Traditional and Western Medicine Tumor,Tangshan People’s Hospital,Tangshan 063001,China;The First Radiochemical Department of Tangshan People’s Hospital,Tangshan 063001,China;Tangshan Lunan District Health Bureau Tangshan City,Tangshan 063001,China)

机构地区：[1]唐山市人民医院中医科,河北唐山063001 [2]唐山市人民医院中西医结合肿瘤科,河北唐山063001 [3]唐山市人民医院放化一科,河北唐山063001 [4]唐山市路南区卫生健康局,河北唐山063001

出　　处：《标记免疫分析与临床》2023年第7期1211-1216,共6页Labeled Immunoassays and Clinical Medicine

基　　金：河北省中医药管理局科研计划项目(编号:2020412)。

摘　　要：目的研究circDOCK1靶向miR-3194-3p对人肺癌A549细胞增殖、迁移和侵袭的影响。方法2019年1月至2021年12月收集唐山市人民医院收治的41例肺癌患者的肺癌组织和癌旁组织,RT-qPCR检测circDOCK1、miR-3194-3p在肺癌组织和癌旁组织中表达。体外培养人肺癌A549细胞,分为si-circDOCK1组、si-NC组、miR-3194-3p组、miR-NC组、pcDNA组、pcDNA-circDOCK1组、si-circDOCK1+anti-miR-NC组、si-circDOCK1+anti-miR-3194-3p组,CCK-8实验、划痕愈合实验、平板克隆实验、Transwell法评估A549细胞体外增殖、迁移、克隆和侵袭能力。双荧光素酶报告实验分析circDOCK1和miR-3194-3p靶向关系。结果与癌旁组织比较,肺癌组织中circDOCK1表达升高(P<0.05),miR-3194-3p表达降低(P<0.05)。干扰circDOCK1或过表达miR-3194-3p可降低A549细胞活力、划痕愈合率、克隆形成数和侵袭细胞数(P<0.05)。抑制miR-3194-3p表达可减弱干扰circDOCK1对A549细胞增殖、迁移和侵袭的抑制作用(P<0.05)。circDOCK1靶向负调控miR-3194-3p表达。结论干扰circDOCK1可通过靶向上调miR-3194-3p表达抑制肺癌A549细胞增殖、迁移和侵袭。Objective To study the effect of circDOCK1 targeting miR-3194-3p on the proliferation,migration and invasion of human lung cancer A549 cells.Methods From January,2019 to December,2021,41 lung cancer patients admitted to Tangshan People’s Hospital were collected for lung cancer tissue and adjacent tissues.RT-qPCR was applied to examine expressions of circDOCK1 and miR-3194-3p in lung cancer tissues and adjacent tissues.Human lung cancer A549 cells were cultured in vitro and divided into the si-circDOCK1 group,si-NC group,miR-3194-3p group,miR-NC group,pcDNA group,pcDNA-circDOCK1 group,si-circDOCK1+anti-miR-NC group,and si-circDOCK1+anti-miR-3194-3p group.CCK-8,scratch healing,plate cloning and trans-well assays were performed to evaluate cells’proliferation,migration,cloning and invasion ability in vitro.Dual luciferase report assay was used to investigate the targeting relationship between circDOCK1 and miR-3194-3p.Results Compared with adjacent tissues,circDOCK1 expression was increased in lung cancer tissues(P<0.05),while miR-3194-3p expression was decreased(P<0.05).Interference with circDOCK1 or overexpression of miR-3194-3p decreased cells’viability,scratch healing rate,number of clones and number of invasive cells(P<0.05).Inhibition of miR-3194-3p expression weakened the inhibition of circDOCK1 interference on proliferation,migration and invasion of A549 cells(P<0.05).circDOCK1 targeting negatively regulated the expression of miR-3194-3p.Conclusion Interfering with circDOCK1 can inhibit cells’proliferation,migration and invasion in lung cancer A549 cells by targeting and up-regulating miR-3194-3p expression.

关 键 词：肺癌 circDOCK1 miR-3194-3p 增殖 迁移 

分 类 号：R734.2[医药卫生—肿瘤]