miR-183-5p靶向FOXN2促进前列腺癌细胞PC3增殖及侵袭的机制研究  

miR-183-5p promote the proliferation and invasion of prostate cancer cell PC3 by targeting FOXN2 in colorectal cancer

作　　者：杨帅[1] 汪盛[1] 陈志军[1] 伍宏亮[1] 关翰 YANG Shuai;WANG Sheng;CHEN Zhi-jun;WU Hong-liang;GUAN Han(Department of Urology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医学院第一附属医院泌尿外科,安徽蚌埠233000

出　　处：《川北医学院学报》2023年第9期1155-1160,共6页Journal of North Sichuan Medical College

基　　金：安徽省高校自然科学重点项目(KJ2021A0777);安徽省高校自然科学重点项目(KJ2019A0355);安徽省自然科学基金(2008085QH358)。

摘　　要：目的:探究miR-183-5p对PC3细胞生物学功能的影响和作用机制。方法:从GEO数据库中筛选在前列腺癌中显著高表达的miR-183-5p,在PC3细胞中过表达miR-183-5p作为miR-183-5p mimics组;将转染空白质粒的PC3细胞作为miR-NC组,通过细胞克隆、5-乙炔基-2’-脱氧尿苷(EdU)实验、噻唑蓝(MTT)、细胞划痕、Transwell小室迁移及侵袭实验检测miR-183-5p mimics组与miR-NC组前列腺癌细胞PC3生物学功能的影响。在PC3细胞中稳定过表达miR-183-5p进行皮下成瘤实验观察miR-183-5p在体内对细胞增殖能力的影响。通过预测软件筛选出叉头框蛋白(FOXN2)作为miR-183-5p的下游靶基因并检测miR-183-5p对其蛋白表达的影响。在PC3细胞中转染FOXN2过表达质粒,共转染miR-183-5p后进行回复实验验证miR-183-5p通过靶向FOXN2促进PC3细胞增殖及侵袭。结果:与miR-NC组比较,miR-183-5p mimics组细胞增殖、迁移及侵袭能力均更高(P<0.05),且其在体内亦可促进瘤体生长(P<0.05)。miR-183-5p组与靶基因FOXN2表达量高于miR-NC组(P<0.05),转染FOXN2质粒后可逆转miR-183-5p mimics对细胞增殖及侵袭的促进作用。结论:miR-183-5p可通过靶向FOXN2促进前列腺癌细胞PC3的增殖和侵袭能力。Objective:To explore the effect and mechanism of miR-183-5p on the biological function of PC3 cells.Methods:GEO database screened miR-183-5p,which was significantly highly expressed in prostate cancer,for research,overexpressed miR-183-5p in PC3 cells was selected as the miR-183-5p mimics group,PC3 cells transfected with blank plasmids were selected as miR-NC group.Cell cloning,EdU experiment,MTT,cell scratch,Transwell chamber migration and Invasion assay were used to detect the effects of miR-183-5p mimics group and miR-NC group on the biological function of prostate cancer cell PC3.Stably overexpressing miR-183-5p in PC3 cells for subcutaneous tumorigenesis experiments to observe the effect of miR-183-5p on cell proliferation in vivo.Forkhead box N2(FOXN2)was screened out by the prediction software as the downstream target gene of miR-183-5p and the effect of miR-183-5p on its protein expression was detected.The FOXN2 overexpression plasmid was transfected in PC3 cells,and miR-183-5p was co-transfected,and the recovery experiment was carried out to verify that miR-183-5p promoted the proliferation and invasion of PC3 cells by targeting FOXN2.Results:Compared with the miR-NC group,the miR-183-5p mimics group showed higher cell proliferation,migration and invasion(P<0.05),and it could promote tumor growth in vivo(P<0.05).The expression of the target gene FOXN2 in miR-183-5p group was higher than that in miR-NC group(P<0.05),and the effect of miR-183-5p mimics on cell proliferation and invasion could be reversed after transfection of the FOXN2 plasmid promotion.Conclusion:miR-183-5p can promote the proliferation and invasion of prostate cancer cell PC3 by targeting FOXN2.

关 键 词：前列腺癌 miR-183-5p 叉头框蛋白 增殖 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]