健脾活血方对非酒精性脂肪性肝炎大鼠肝脂代谢及炎症因子的影响  

Effect of Jianpi Huoxue formula on hepatic lipid metabolism and inflammatory factors in rats with nonalcoholic steatohepatitis

作　　者：朱德鑫 邵建国[2] 冯程程 陈琳[2] 黄新雨 ZHU De-xin;SHAO Jian-guo;FENG Cheng-cheng(Rudong Hospital of Traditional Chinese Medicine,Nantong City,Jiangsu Province,Nantong 226400,China;不详)

机构地区：[1]江苏省南通市如东县中医院,南通226400 [2]江苏省南通市第三人民医院

出　　处：《山西中医》2023年第10期54-56,共3页Shanxi Journal of Traditional Chinese Medicine

基　　金：江苏省南通市基础研究和民生科技计划指导性项目(编号:JCZ21056)。

摘　　要：目的:观察健脾活血方对非酒精性脂肪性肝炎大鼠肝功能、血脂、血清炎症因子的影响。方法:以高脂饲料制备非酒精性脂肪性肝炎大鼠模型,成功造模后将大鼠分为正常组、模型组、健脾活血方组(高、中、低3个剂量组)。健脾活血方组高、中、低剂量组分别予健脾活血方26.6、13.3、6.65 g/(kg·d)灌胃,模型组和正常组以0.9%NaCl溶液灌胃,8周后检测大鼠肝功能、血脂及血清中炎症因子的水平。结果:高脂饮食后,大鼠ALT、AST、TBIL、TC、TG、LDL-C、IL-1β、IL-18、INF-α含量均显著升高(P﹤0.01),HDL-C显著下降(P﹤0.01)。与模型组比较,健脾活血方高、中、低剂量组血清AST、TG、IL-1β、IL-18、INF-α含量均显著下降,HDL-C显著升高(P﹤0.05或P﹤0.01),健脾活血方高、中剂量组血清ALT、TC、LDL-C含量均显著性下降(P﹤0.05或P﹤0.01);健脾活血方高剂量组TBIL显著下降(P﹤0.05)。结论:健脾活血方可改善非酒精性脂肪性肝炎大鼠肝功能、血脂,降低炎症因子水平。Objective:To approach the effect of Jianpi Huoxue formula on liver function,blood lipid and serum inflammatory factors in rats with non-alcoholic steatohepatitis(NASH).Methods:The rat model of NASH was established with high-fat diet.After successful modeling,the rats were divided into normal group,model group,Jianpi Huoxue formula group(high-,medium-and low-dose treatment group).The high-,medium-and low-dose group of Jianpi Huoxue formula were given 26.6,13.3 and 6.65 g/(kg·d)of Jianpi Huoxue formula by gavage respectively,while the model group and normal group were given 0.9%NaCl solution by gavage respectively.The levels of liver function,blood lipid and serum inflammatory factors of rats were detected after 8 weeks.Results:After high-fat diet,the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),interleukin-1β(IL-1β),interleukin-18(IL-18)and Interferon-α(INF-α)in rats were significantly increased(P﹤0.01),while high density lipoprotein cholesterol(HDL-C)was significantly decreased(P﹤0.01).Compared with model group,in high-,medium-and low-dose group of Jianpi Huoxue formula,serum AST,TG,IL-1β,IL-18 and INF-αwere significantly decreased,HDL-C was significantly increased(P﹤0.05 or P﹤0.01);in high-and medium-dose group of Jianpi Huoxue formula,serum ALT,TC and LDL-C were significantly decreased(P﹤0.05 or P﹤0.01);in high-dose group of Jianpi Huoxue formula,TBIL was significantly decreased(P﹤0.05).Conclusion:Jianpi Huoxue formula can improve liver function,blood lipid of rats with NASH,and reduce the level of inflammatory factors in rats with NASH.

关 键 词：非酒精性脂肪性肝炎 肝脂代谢 炎症因子 健脾活血方 实验研究 

分 类 号：R575.29[医药卫生—消化系统]