间歇性冷刺激对高脂饮食小鼠的血清脂质相关指标、肝脏组织形态及肝脏脂质合成相关基因和蛋白表达的影响  

Effects of Intermittent Cold Stimulation on Serum Lipid⁃Related Indexes,Liver Tissue Morphology and Expression of Genes and Proteins Related to Liver Lipid Synthesis in Mice Fed High Fat Diets

作　　者：李晓双 刘宏睿 付鹏飞 张萌[1] 寇乐乐 张博熙 徐彬 李士泽[1] LI Xiaoshuang;LIU Hongrui;FU Pengfei;ZHANG Meng;KOU Lele;ZHANG Boxi;XU Bin;LI Shize(Key Laboratory of Bovine Disease Control in Northeast Cold Region,Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163000,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,农村农业部东北寒区牛病防治重点实验室,大庆163000

出　　处：《动物营养学报》2023年第9期6004-6012,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金面上项目(31972637)。

摘　　要：为了探究间歇性冷刺激对高脂饮食小鼠血清脂质相关指标、肝脏组织形态以及肝脏脂质合成的影响,试验选择5周龄的雄性C57BL/6小鼠48只,随机分为常温对照组、常温高脂组、冷刺激组和冷刺激高脂组,每组3个重复,每个重复4只。常温组小鼠饲养温度为(26±2)℃,冷刺激组小鼠饲养温度为4℃,试验期30 d(每天6 h)。处理结束后采集各组小鼠的肝脏组织和血清样本,通过苏木素-伊红(HE)染色、蛋白质免疫印迹(Western blot)、实时荧光定量PCR(qRT-PCR)等方法,对小鼠肝脏形态结构和脂质合成相关指标进行检测。结果显示:1)饲喂高脂饲粮的小鼠在14周时,体重极显著高于常温对照组(P<0.01)。2)HE结果表明,常温对照组小鼠肝脏肝小叶结构清晰,肝索排列规则有序,未见水肿变性和坏死,未见炎细胞;冷刺激组小鼠的肝脏组织出现颗粒变性,炎性细胞浸润,并观察到充血和出血现象;常温高脂组小鼠肝脏可见大小不一的圆形空泡,发生大泡性脂肪变;冷刺激高脂组小鼠肝脏肝小叶排列紊乱,肝细胞水肿可见空泡变性,炎性细胞浸润。3)小鼠高脂造模后,常温高脂组小鼠血清中血脂相关指标甘油三酯(TG)、游离脂肪酸(NEFA)、总胆固醇(T-CHO)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)的含量均极显著升高(P<0.01)。4)冷刺激组小鼠血清中TG、NEFA、T-CHO、HDL-C、LDL-C的含量极显著低于对照组(P<0.01)。5)常温高脂组小鼠在冷刺激后血清中TG、NEFA、T-CHO、HDL-C、LDL-C的含量极显著低于高脂组(P<0.01)。6)小鼠高脂造模后,常温高脂组小鼠肝脏脂质合成相关[脂肪酸合酶(FASN)、乙酰辅酶A羧化酶1(ACC1)、固醇元件结合蛋白1c(SREBP1c)、ATP-柠檬酸裂解酶(ACLY)]基因和蛋白表达水平极显著升高(P<0.01);小鼠间歇性冷刺激后,冷刺激组小鼠肝脏ACC1蛋白表达水平极显著升高(P<0.01),FASN蛋白表达水平极显著降低(P<0.01);与To investigate the effects of intermittent cold stimulation on liver tissue morphology,serum lipid in-dexes and liver lipid synthesis in obese mice,48 male C57BL/6 mice at 5 weeks of age were randomly divided into room temperature control group,room temperature high fat group,cold stimulation group,and cold stimu-lation high fat group.There were three replicates in each group,and there were four mice in each replicate.The feeding temperature of mice in the room temperature group was(26±2)℃,and the feeding temperature of the mice in the cold stimulation group was 4℃for 30 days(6 h per day).After the end of treatment,liver tissue and serum samples of each group of mice were collected,and the liver morphological structure and lipid syn-thesis related indexes of mice were detected by hematoxylin-eosin(HE)staining,Western blot,and real-time PCR(qRT-PCR).The results showed as follows:1)the weight of mice fed high fat diet was significantly higher than that in the control group at 14 weeks(P<0.01).2)The results of HE showed that the liver lobules in the room temperature control group had a clear structure,regular and orderly arrangement of liver cords,no edema degeneration and necrosis,and no inflammatory cells;the liver tissue of mice in the cold stimulation group showed granular degeneration,inflammatory cell infiltration,and hyperemia and bleeding were ob-served;round vacuoles of different sizes and bullous steatosis occurred in the livers of mice in the room temper-ature high fat group;cold stimulation high fat group mice with disordered hepatic lobular arrangement,hepato-cyte edema showed vacuolar degeneration,inflammatory cell infiltration.3)After high-fat modeling,the ser-um lipid-related indexes of triglyceride(TG),non-esterified fatty acid(NEFA),total cholesterol(T-CHO),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)contents was sig-nificantly increased in high fat group(P<0.01).4)The contents of TG,NEFA,T-CHO,HDL-C and LDL-C in serum of mice in the cold stimulation g

关 键 词：间歇性冷刺激 高脂饮食 肝脏 脂肪合成 

分 类 号：S816[农业科学—饲料科学]