Identification and epitope mapping of anti-p72 single-chain antibody against African swine fever virus based on phage display antibody library  

作　　者：SONG Jin-xing WANG Meng-xiang ZHANG Yi-xuan WAN Bo DU Yong-kun ZHUANG Guo-qing LI Zi-bin QIAO Song-lin GENG Rui WU Ya-nan ZHANG Gai-ping 

机构地区：[1]College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,P.R.China [2]International Joint Research Center of National Animal Immunology,Zhengzhou 450046,P.R.China [3]College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,P.R.China [4]Longhu Laboratory,Zhengzhou 450046,P.R.China [5]School of Advanced Agricultural Sciences,Peking University,Beijing 100871,P.R.China [6]Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第9期2834-2847,共14页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31941001 and 32002292);the Major Science and Technology Project of Henan Province,China(221100110600);the Natural Science Foundation of Henan Province(202300410199).

摘　　要：African swine fever virus(ASFV)is a lethal pathogen that causes severe threats to the global swine industry and it has already had catastrophic socio-economic effects.To date,no licensed prophylactic vaccine exists.Limited knowledge exists about the major immunogens of ASFV and the epitope mapping of the key antigens.As such,there is a considerable requirement to understand the functional monoclonal antibodies(mAbs)and the epitope mapping may be of utmost importance in our understanding of immune responses and designing improved vaccines,therapeutics,and diagnostics.In this study,we generated an ASFV antibody phage-display library from ASFV convalescent swine PBMCs,further screened a specific ASFV major capsid protein(p72)single-chain antibody and fused with an IgG Fc fragment(scFv-83-Fc),which is a specific recognition antibody against ASFV Pig/HLJ/2018 strain.Using the scFv-83-Fc mAb,we selected a conserved epitope peptide(221MTGYKH226)of p72 retrieved from a phage-displayed random peptide library.Moreover,flow cytometry and cell uptake experiments demonstrated that the epitope peptide can significantly promote BMDCs maturation in vitro and could be effectively uptaken by DCs,which indicated its potential application in vaccine and diagnostic reagent development.Overall,this study provided a valuable platform for identifying targets for ASFV vaccine development,as well as to facilitate the optimization design of subunit vaccine and diagnostic reagents.

关 键 词：ASFV phage display antibody library single chain antibody p72 EPITOPE 

分 类 号：S852.65[农业科学—基础兽医学]