人表皮生长因子受体2在结直肠癌中的表达及其临床意义  

Expression of epidermal growth factor receptor 2 in colorectal cancer and its clinical significance

作　　者：詹世宁 李菲[1] 卓静[1] 杜丽 王灿 李晓峰[2] ZHAN Shining;LI Fei;ZHUO Jing;DU Li;WANG Can;LI Xiaofeng(Department of Pathology,Xuzhou Cancer Hospital,Jiangsu Province,Xuzhou221000,China;Department of Radiology,Xuzhou Hospital Affiliated to Jiangsu University,Jiangsu Province,Xuzhou221000,China)

机构地区：[1]江苏省徐州市肿瘤医院病理科,江苏徐州221000 [2]江苏大学附属徐州医院影像科,江苏徐州221000

出　　处：《中国当代医药》2023年第28期4-7,F0003,共5页China Modern Medicine

基　　金：江苏大学临床医学科技发展基金项目(JLY2021082)。

摘　　要：目的探讨人表皮生长因子受体2(HER-2)在结直肠癌中的表达及其与临床病理特征的关系。方法选取2019年4月至2022年1月徐州市肿瘤医院病理科100例结直肠癌标本设为结直肠癌组,将其中距肿瘤5 cm以上的60例正常肠黏膜设为正常肠黏膜组,另选取同期的60例结直肠腺瘤标本设为结直肠腺瘤组。三组均采用免疫组织化学(IHC)方法和荧光原位杂交(FISH)技术检测。分析HER-2在三组中的表达情况,比较三组HER-2(2+)基因扩增结果、三组HER-2的表达、结直肠癌不同临床病理特征的HER-2表达。结果结直肠癌组中,HER-2(3+)15例,HER-2(2+)45例,HER-2(0)或(1+)40例。结直肠腺瘤组中,HER-2(3+)7例,HER-2(2+)22例,HER-2(0)或(1+)31例。正常肠黏膜组中HER-2(2+)7例,HER-2(0)或(1+)53例。三组的HER-2(2+)基因扩增结果比较,差异无统计学意义(P>0.05)。结直肠癌组、结直肠腺瘤组的HER-2阳性表达均显著高于正常黏膜组,差异有统计学意义(P<0.05);结直肠癌组与结直肠腺瘤组的HER-2阳性表达比较,差异无统计学意义(P>0.05)。不同性别、年龄、肿瘤直径、TNM分期的HER-2阳性表达比较,差异无统计学意义(P>0.05);“高+中分化”的HER-2阳性表达低于“低分化”,“Tis+T1+T2”浸润深度的HER-2阳性表达低于“T3+T4”浸润深度,有淋巴结转移的HER-2阳性表达高于无淋巴结转移,差异有统计学意义(P<0.05)。结论HER-2在结直肠癌和腺瘤中均存在高表达,与癌组织的分化程度、浸润深度及淋巴结转移有密切关系。Objective To investigate the expression of epidermal growth factor receptor 2(HER-2)in colorectal cancer and its relationship with clinical pathology features.Methods A total of 100 colorectal cancer samples from the Department of Pathology of Xuzhou Cancer Hospital from April 2019 to January 2022 were selected as colorectal cancer group,60 normal intestinal mucosa samples more than 5 cm away from the tumor were selected as normal intestinal mucosa group,and another 60 colorectal adenoma samples from the same period were selected as colorectal adenoma group.Immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)was used to detect the expression of three groups in all patients.The expression of Her-2 in three groups was analyzed,and the results of HER-2(2+)gene amplification,the expression of HER-2 in three groups and the expression of HER-2 in colorectal carcinoma with different clinicopathological characteristics were compared.Results In the colorectal cancer group,there were 15 cases of HER-2(3+),45 cases of HER-2(2+),40 cases of HER-2(0)or(1+).In the colorectal adenoma group,there were 7 cases of HER-2(3+),22 cases of HER-2(2+)and 31 cases of HER-2(0)or(1+).There were 7 cases of HER-2(2+)and 53 cases of HER-2(0)or(1+)in normal colorectal tissues group.There was no significant difference in the amplification of HER-2(2+)gene among the three groups(P>0.05).The expression of HER-2 in colorectal carcinoma and colorectal adenoma was higher than that in normal mucosa,with a statistically significant difference(P<0.05).There was no statistically difference in the expression of HER-2 between the colorectal cancer group and the colorectal adenoma group(P>0.05).There were no significant differences in the expression of HER-2 in different gender,age,tumor diameter and TNM stage(P>0.05).The expression of HER-2 in"high+moderate differentiation"was lower than that in"low differentiation",and the expression of HER-2 in"Tis+T1+T2"was lower than that in"T3+T4",the positive expression of HER-2 in patients with lym

关 键 词：结直肠癌 腺瘤 正常肠黏膜 人表皮生长因子受体2 免疫组织化学 荧光原位杂交 

分 类 号：R735.3[医药卫生—肿瘤]