桤木Genomic-SSR与EST-SSR分子标记的差异分析  

Genetic Differences Revealed by Genomic-SSR and EST-SSR Markers in Alnus cremastogyne

作　　者：王泽亮[1] 杨勇智[1] 杜晋城[1] 陈炙[1] 黄振[1] 郭洪英[1,2] WANG Zeliang;YANG Yongzhi;DU Jincheng;CHEN Zhi;HUANG Zhen;GUO Hongying(Sichuan Academy of Forestry,Chengdu 610081,China;Sichuan Academy of Grassland Sciences,Chengdu 611731,China)

机构地区：[1]四川省林业科学研究院,四川成都610081 [2]四川省草原科学研究院,四川成都611731

出　　处：《四川林业科技》2023年第4期36-42,共7页Journal of Sichuan Forestry Science and Technology

基　　金：世界银行贷款长江流域上游森林生态系统恢复项目(2019-510000-02-01-400761);四川省科技计划项目(2021YFYZ0032)。

摘　　要：桤木(Alnus cremastogyne)为非豆科固氮树种,也是我国最重要的一个桤木属特有种,具有重要的生态功能。本文分析了桤木基因组SSR与EST-SSR两种来源标记的遗传差异,结果显示EST-SSR的平均等位基因数与平均有效等位基因数高于Genomic-SSR,而对平均观察杂合度与平均期望杂合度来说,Genomic-SSR高于EST-SSR,聚类分析显示Genomic-SSR与EST-SSR在小的分群上有差异,说明Genomic-SSR与ESTSSR标记在解析遗传多样性与遗传关系方面有一定差异,综合两种标记可以获得更加客观的结果。As a non-leguminous and nitrogen-fixing tree species,Alnus cremastogyne is also the most important endemic species of Alnus in China,which has important ecological functions.In this paper,the genetic differences of Genomic-SSR and EST-SSR markers in Alnus cremastogyne genome were analyzed.The results showed that the average number of alleles and the average number of effective alleles with EST-SSR were higher than those of genomic-SSR,while the average observed heterozygosity and average expected heterozygosity were higher than those of EST-SSR.Cluster analysis showed that there were differences between genomic-SSR and EST-SSR in small groups,which indicated that there were some differences between Genomic-SSR and EST-SSR in analyzing genetic diversity and genetic relationship to a certain extent,and more objective results could be obtained by combining the two marker methods.

关 键 词：桤木 简单序列重复 遗传多样性 多倍体 GenoDive 

分 类 号：S792.14[农业科学—林木遗传育种]