虾青素通过调节Klotho表达和Wnt/β-Catenin信号通路对多囊卵巢综合征大鼠的改善作用  

The improvement effect of astaxanthin on rats with polycystic ovary syndrome by regulating Klotho expression and Wnt/β-Catenin signaling pathway

作　　者：宋玉 朱争艳 黄桓 李春 鲁晶泉 刘和宇 胡丽娜 SONG Yu;ZHU Zhengyan;HUANG Huan;LI Chun;LU Jingquan;LIU Heyu;HU Lina(Department of Gynecology,Wuhan Third Hospital,Wuhan 430000,China)

机构地区：[1]武汉市第三医院妇科,430000

出　　处：《天津医药》2023年第10期1098-1103,共6页Tianjin Medical Journal

基　　金：武汉市医学科研项目(WZ21Z01)。

摘　　要：目的研究虾青素调节Klotho表达和Wnt/β-Catenin信号通路对多囊卵巢综合征(PCOS)大鼠的改善作用及机制。方法采用来曲唑溶液灌胃的方法构建30只PCOS模型大鼠,随机分为6组:模型组、虾青素低剂量组(50 mg/kg)、虾青素中剂量组(100 mg/kg)、虾青素高剂量组(200 mg/kg)、虾青素高剂量+空载组、虾青素高剂量+Klotho敲低组,每组5只;另取5只SD大鼠作为对照组。分组处理后测定各组大鼠体质量与卵巢质量。取SD大鼠以同样方法造模并随机分为:模型组、虾青素组(200 mg/kg)、虾青素+Klotho敲低组、虾青素+氯化锂组、虾青素+PD98059(MEK抑制剂)组,每组9只,另取9只SD大鼠作为对照组。分组处理后,测定各组大鼠体质量、卵巢质量与体积、卵巢组织囊状卵泡数量,血清卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)水平及炎性因子前列腺素E2(PGE2)、白细胞介素(IL)-17、肿瘤坏死因子-α(TNF-α)、IL-18水平,卵巢组织Klotho蛋白表达和Wnt/β-Catenin、MEK/ERK信号相关蛋白表达。结果与对照组比较,模型组体质量与卵巢质量明显升高(P<0.05);与模型组比较,虾青素低剂量组、虾青素中剂量组、虾青素高剂量组的体质量与卵巢质量均降低,并呈剂量依赖性(P<0.05);与虾青素高剂量组比较,虾青素高剂量+Klotho敲低组体质量与卵巢质量升高(P<0.05),虾青素高剂量+空载组各指标无明显变化。与对照组比较,模型组血清FSH水平和卵巢组织Klotho、p-MEK、p-ERK1/2蛋白表达降低(P<0.05),体质量、卵巢质量与体积、囊状卵泡数量、血清LH、T、PGE2、IL-17、TNF-α及IL-18水平、卵巢组织Wnt1与β-Catenin蛋白表达明显升高(P<0.05);与模型组比较,虾青素组血清FSH水平和卵巢组织Klotho、p-MEK、p-ERK1/2蛋白表达升高(P<0.05),体质量、卵巢质量与体积、囊状卵泡数量、血清LH、T、PGE2、IL-17、TNF-α及IL-18水平、卵巢组织Wnt1与β-Catenin蛋白表达降低(P<0.05);敲低KlObjective To study the improvement mechanism of astaxanthin in rats with polycystic ovary syndrome(PCOS)by regulating Klotho expression and Wnt/β-Catenin signaling pathway.Methods Thirty PCOS model rats were constructed by intragastric administration of letrozole,and rats were randomly grouped into 6 groups:the model group,the low-dose astaxanthin group(50 mg/kg),the middle-dose astaxanthin groupm(100 mg/kg),the high-dose astaxanthin group(200 mg/kg),the high-dose astaxanthin+no-load group and the high-dose astaxanthin+Klotho knockdown group,with 5 rats in each group.Another 5 SD rats were taken as the control group.After treatment,the body weight and ovarian weight of rats in each group were measured.Forty-five SD rats were modeled in the same way and randomly grouped into:the model group,the astaxanthin group(200 mg/kg),the astaxanthin+Klotho knockdown group,the astaxanthin+lithium chloride group and the astaxanthin+PD98059(MEK inhibitor)group,with 9 rats in each group.Another 9 SD rats were taken as the control group.After treatment,data of body weight,ovarian weight and volume,the number of cystic follicles in ovarian tissue,levels of serum hormones including follicle-stimulating hormone(FSH),luteinizing hormone(LH),testosterone(T)and levels of inflammatory factors prostaglandin 2(PGE2),interleukin(IL)-17,tumor necrosis factorα(TNFα),and IL-18,the expression of Klotho protein and the expression of Wnt/β-Catenin and MEK/ERK signal-related proteins in ovarian tissue were detected in rats of each group.Results Compared with the control group,body weight and ovarian weight were obviously increased in the model group(P<0.05).Compared with the model group,body weight and ovarian weight were all decreased in the low-dose astaxanthin group,the middle-dose astaxanthin group and the high-dose astaxanthin group in a dose-dependent manner(P<0.05).Compared with the high-dose astaxanthin group,body mass and ovarian mass of rats were increased in the high-dose astaxanthin+Klotho knockdown group(P<0.05).There was no sign

关 键 词：多囊卵巢综合征 WNT信号通路 MAP激酶信号系统 虾青素 KLOTHO 

分 类 号：R711.75[医药卫生—妇产科学]