Establishment,Characterization and Expression Pattern of a Spleen Cell Line,SMSP,Derived from Turbot(Scophthalmus maximus)  

作　　者：XUE Ting LIU Yiping GE Xuefeng LI Chao 

机构地区：[1]School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Journal of Ocean University of China》2023年第5期1403-1411,共9页中国海洋大学学报（英文版）

基　　金：the National Natural Science Foundation of China(No.31902403);the Young Experts of Taishan Scholars(No.tsqn201909130);the advanced Talents Foundation of QAU grant(No.663-1120029);the Shandong Technical System of Fish Industry(No.SDAIT-12-03);the Breeding Plan of Shandong Provincial Qingchuang Research Team(2019),China。

摘　　要：A turbot(Scophthalmus maximus)cell line named SMSP was obtained from the spleen.The origin of the cells was identified by morphology,chromosome number and COI gene.The optimal basic medium,serum concentration and growth temperature of the cells were detected.SMSP cell line is mainly composed of fibroblast-like cells.Most of the SMSP cells contained 44 chromosomes,and the sequence of COI gene confirmed that the cells were originated from turbot.The optimal culture conditions were 24℃,DMEM+10%FBS.The cell line had high transfection efficiency for siRNA and plasmid.After stimulation with lipopolysaccharide(LPS)or poly(I:C),the expressions of immune-related genes such as TNF-β,IL-12s,IL-10 and IL-1βwere up-regulated significantly in the early stage(P<0.05).This study will provide a model for exploring immune mechanism of turbot against pathogen in vitro.

关 键 词：Scophthalmus maximus cell line SPLEEN pathogen-associated molecular patterns IMMUNITY 

分 类 号：S917.4[农业科学—水产科学]