杜仲叶提取物绿色合成纳米铂颗粒及其美白作用  

Green synthesis of platinum nanoparticles by Eucommia ulmoides Oliver leaf extract and its whitening effect

作　　者：张义森 程硕 周娟娟 贾会领 王军[2] 陈雪[2] 吴丽芳 ZHANG Yisen;CHENG Shuo;ZHOU Juanjuan;JIA Huiling;WANG Jun;CHEN Xue;WU Lifang(School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China;The Center for Ion Beam Bioengineering&Green Agriculture,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China)

机构地区：[1]安徽医科大学基础医学院,安徽合肥230032 [2]中国科学院合肥物质科学研究院离子束生物工程与绿色农业研究中心,安徽合肥230031

出　　处：《轻工学报》2023年第5期119-126,共8页Journal of Light Industry

基　　金：中国科学院战略生物资源计划项目(KFJ-BRP-007-014)。

摘　　要：以杜仲叶提取物为还原剂和稳定剂,采用一步法绿色合成纳米铂颗粒(PtNPs),通过单因素试验分析反应条件对PtNPs颜色和浓度的影响,并研究PtNPs对酪氨酸酶活性的影响。结果表明:在一定范围内改变反应条件可调控合成PtNPs的颜色和浓度,适宜反应条件为反应时间60 min、反应温度90℃、氯铂酸浓度3.5 mmol/L、杜仲叶提取物质量浓度10.0 mg/mL,在该条件下制备的PtNPs粒径平均尺寸为(2.27±0.65) nm,具有面心立方结构,分布较均匀;PtNPs对体外蘑菇酪氨酸酶活性有较强的抑制作用,IC50为(6.98±0.76)μg/mL,显著降低了A375细胞内酪氨酸酶活性和UVB诱导的黑色素含量,可达到美白皮肤的效果。Platinum nanoparticles(PtNPs)were synthesized by one-step green method using Eucommia ulmoides Oliver leaf extract as reducer and stabilizer.The effects of reaction conditions on the color and absorbance concentration of PtNPs were analyzed by single factor test,and the effect of PtNPs on tyrosinase activity was studied.The results showed that the color and yield of PtNPs could be controlled by changing the reaction conditions in a certain range.The suitable reaction conditions of each factor were reaction time of 60 min,reaction temperature of 90℃,chloroplatinic acid concentration of 3.5 mmol/L,Eucommia ulmoides Oliver leaf extract concentration of 10.0 mg/mL.Under these conditions,the average size of prepared PtNPs was(2.27±0.65)nm,with face-centered cubic structure and dispersed distribution.PtNPs had a strong inhibitory effect on tyrosinase activity in vitro,IC 50 value was(6.98±0.76)μg/mL,which significantly reduced tyrosinase activity and melanin production content induced by UVB in A375 cells,and could achieve the effect of whitening skin.

关 键 词：杜仲叶提取物 纳米铂颗粒 绿色合成 酪氨酸酶活性 

分 类 号：TS974.1[轻工技术与工程] Q599[生物学—生物化学]