基于网络药理学的火炭母、凤尾草治疗溃疡性结肠炎的潜在机制研究  

Study on the Potential Mechanism of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir in Treating Ulcerative Coliti Based on Network Pharmacology

作　　者：陈钰凤 范明 马玉婷[1] CHEN Yufeng;FAN Ming;MA Yuting(The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine,Guangzhou 510000,China;Guangdong Second Hospital of Traditional Chinese Medicine,Guangzhou 510000,China)

机构地区：[1]广州中医药大学第五临床医学院,广东广州510000 [2]广东省第二中医院,广东广州510000

出　　处：《中国中医药图书情报杂志》2023年第6期59-64,共6页Chinese Journal of Library and Information Science for Traditional Chinese Medicine

摘　　要：目的 探索中药火炭母、凤尾草治疗溃疡性结肠炎(UC)的有效成分及其作用机制。方法 通过检索文献收集火炭母、凤尾草的主要成分,通过TCMSP、SwissADME数据库对成分进行筛选,SwissTargetPrediction数据库获得活性成分的作用靶点;使用Cytoscape3.9.1构建“成分-靶点”网络,筛选核心成分。通过GeneCards、OMIM数据库筛选UC靶点;使用STRING数据库、Cytoscape3.9.1软件构建交集靶点蛋白相互作用网络;应用DAVID数据库进行GO功能和KEGG通路富集分析。通过AutoDock软件对核心成分与核心靶点进行分子对接验证。结果 得到火炭母、凤尾草23个活性成分,其中木犀草素、山柰酚、槲皮素、异鼠李素、芹菜素等为其抗UC核心成分。得到火炭母-凤尾草抗UC潜在靶点146个,核心靶点涉及MAPK3、SRC、MAPK1、AKT1、LCK等。GO功能及KEGG通路富集分析结果显示,火炭母-凤尾草抗UC作用机制主要与癌症通路、EGFR酪氨酸激酶抑制剂、PI3K-Akt通路、HIF-1通路、MAPK通路有关。分子对接显示,火炭母-凤尾草核心成分与核心靶点结合较好。结论 火炭母、凤尾草可通过MAPK3、SRC、MAPK1、AKT1、LCK等靶点作用于EGFR、癌症通路、PI3K-Akt、MAPK信号通路等,发挥抗炎作用。Objective To study the effective components and mechanism of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir for the treatment of ulcerative colitis(UC).Methods The main active components of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir were retrieved through literature collection.By screening components through TCMSP and SwissADME databases,the SwissTargetPrediction platform obtained the target of action of the active components;a“component target”network was built using Cytoscape 3.9.1 to screen core components.UC targets were screened through GeneCards and OMIM databases;STRING database and Cytoscape 3.9.1 software were used to construct a protein protein interaction network for intersection targets;the DAVID database was used for GO function and KEGG pathway enrichment analysis.Molecular docking verification of core components and core targets was carried out through AutoDock software.Results Totally 23 active components of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir were obtained,of which wood rhinoplasses,yam phenols,citrin,alien lipin,celery,etc.were the core components of anti-ulcerative colitis.146 potential targets of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir were obtained,including MAPK3,SRC,MAPK1,AKT1,LCK,etc.The analysis of the GO function and KEGG pathway enrichment showed that the mechanism of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir of anti-ulcerative colitis was mainly related to the cancer pathway,the EGFR tyrosine kinase inhibitor,the PI3K-Akt signaling pathway,the HIF-1 signaling pathway,and the MAPK signaling pathway and other pathways.The molecular docking showed that the active components of Persicaria chinensis(L.)H.Gross and Pteris multifda Poir had good docking with core targets.Conclusion Persicaria chinensis(L.)H.Gross and Pteris multifda Poir can exert anti-inflammatory effects by targeting EGFR,cancer pathway,PI3K-Akt,MAPK signaling pathway,etc.through MAPK3,SRC,MAPK1,AKT1,LCK,etc.

关 键 词：火炭母 凤尾草 溃疡性结肠炎 网络药理学 分子对接 

分 类 号：R271.94[医药卫生—中西医结合]