A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination,protein labeling,and mitochondrial staining  

作　　者：Xinyuan He Huilin Xie Lianrui Hu Pengchao Liu Changhuo Xu Wei He Wutong Du Siwei Zhang Hao Xing Xinyue Liu Hojeong Park Tsz Shing Cheung Min-Hui Li Ryan T.K.Kwok Jacky W.Y.Lam Jian Lu Ben Zhong Tang 

机构地区：[1]Department of Chemistry,Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction,State Key Laboratory of Molecular Neuroscience,Division of Life Science and Department of Chemical and Biological Engineering,The Hong Kong University of Science and Technology,Kowloon,Hong Kong,China [2]Department of Mechanical Engineering,City University of Hong Kong,Greater Bay Joint Division,Shenyang National Laboratory for Materials Science,Kowloon,Hong Kong,China [3]Chimie ParisTech PSL University Paris,CNRS,Institut de Recherche de Chimie Paris,Paris,France [4]Department of Chemistry,Guangdong-Hongkong-Macou Joint Laboratory of Optoelectronic and Magnetic Functional Materials,The Hong Kong University of Science and Technology,Kowloon,Hong Kong,China [5]Center for Aggregation-Induced Emission,SCUT-HKUST Joint Research Institute,State Key Laboratory of Luminescent Materials and Devices,South China University of Technology,Guangzhou,China [6]School of Science and Engineering,Shenzhen Key Laboratory of Functional Aggregate Materials,The Chinese University of Hong Kong,Shenzhen City,Guangdong,China

出　　处：《Aggregate》2023年第1期250-259,共10页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Number:21788102;Research Grants Council of Hong Kong,Grant/Award Numbers:16307020,16306620,16305518,N_HKUST609/19,C6009-17G,C6014-20w;Innovation and Technology Commission,Grant/Award Numbers:ITC-CNERC14SC01,ITCPD/17-9;Natural Science Foundation of Guangdong Province,Grant/Award Number:201913121205002。

摘　　要：Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research.Despite all the efforts,scientists lack a proper strategy to achieve high selectivity for primary amines,not to mention the requirement of fast response in real applications.Herein,we report a chromone-based aggregation-induced emission(AIE)fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification,and its applications for monitoring bioprocesses of amination and protein labeling.CMVMN is AIE-active and capable of solid-state sensing.Thus,its electrospun films are manufactured for visualization of amine diffusion and leakage process.CMVMN also shows good biocompatibility and potential mitochondria-staining ability,which provides new insight for organelle-staining probe design.Combined with its facile synthesis and good reversibility,CMVMN would not only show wide potential applications in biology,but also offer new possibilities for molecular engineering.

关 键 词：aggregation-induced emission(AIE) BIOCONJUGATION mitochondria-staining primary amines protein labeling 

分 类 号：TQ246[化学工程—有机化工]