铜绿假单胞菌感染后转化生长因子-β1对人肺上皮Beas-2B细胞凋亡及周期的影响  

Influence of transforming growth factor-β1 on Beas-2B apoptosis and cell cycle after Pseudomonas aeruginosa infection

作　　者：张丹 高念顺 孙续国[1] ZHANG Dan;GAO Nian-shun;SUN Xu-guo(Tianjin Medical University,Tianjin 300203,China;不详)

机构地区：[1]天津医科大学医学检验学院,天津300203 [2]三河市儿童医院检验科,河北三河065201 [3]合肥中核中同蓝博医学检验实验室有限公司,安徽合肥230041

出　　处：《中华医院感染学杂志》2023年第17期2566-2569,共4页Chinese Journal of Nosocomiology

基　　金：天津市科技局基金资助项目(2020065)。

摘　　要：目的探究铜绿假单胞菌(PA)感染后转化生长因子-β1(TGF-β1)对人肺上皮Beas-2B细胞凋亡和周期的影响.方法铜绿假单胞菌感染人肺上皮Beas-2B细胞,采用酶联免疫吸附测定法检测Beas-2B细胞感染前后肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)表达水平;流式细胞仪检测感染前后Beas-2B细胞周期变化;实时荧光定量聚合酶链式反应法(qRT-PCR)和蛋白印迹法(WB)检测Beas-2B细胞感染前后TGF-β1信号通路相关基因和蛋白相对表达量.结果与对照组比较,感染组细胞TNF-α和IL-6表达水平均升高(P<0.05);细胞凋亡坏死率和S期比例均增加(P<0.05);TGF-β1和Smad2基因和蛋白表达均升高,Smad7基因和蛋白表达均降低.结论铜绿假单胞菌感染后激发人肺上皮细胞Beas-2B细胞炎症反应,将细胞周期阻滞于S期,抑制细胞的分裂与增殖,促进细胞凋亡.OBJECTIVE To observe the influence of transforming growth factor-β1(TGF-β1)on Beas-2B apoptosis and cell cycle after Pseudomonas aeruginosa infection.METHODS The human lung epithelial cells Beas-2B were infected with P.aeruginosa.The expression levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay before and after the Beas-2B cells were infected.The change of Beas-2B cell cycle was detected by flow cytometry before and after the infection,and the relative expression levels of TGF-β1 signaling pathway-related genes and proteins were detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)and Western blot(WB)before and after the Beas-2B were infected.RESULTS The expression levels of TNF-αand IL-6 in the cells were higher in the infection group than in the control group(P<0.05).The apoptosis rate and S-phase ratio of the infection group were higher than those of the control group(P<0.05).The expression levels of TGF-β1 and Smad2 genes and proteins of the infection group were higher than those of the control group;the expression levels of Smad7 gene and protein of the infection group were lower than those of the control group.CONCLUSION The P.aeruginosa infection may trigger the inflammatory response of the human lung epithelial cell Beas-2B,block the cell cycle in S phase and inhibit the cell division and proliferation,promotethecell apoptosis.

关 键 词：铜绿假单胞菌 人肺上皮细胞Beas-2B 细胞凋亡 细胞周期 转化生长因子-Β1 

分 类 号：R-33[医药卫生]