结直肠癌术后腹腔感染的风险预测模型构建及Hippo信号通路基因表达  

Establishment of risk prediction model for postoperative abdominal infection in colorectal cancer patients and expressions of Hippo signaling pathway genes

作　　者：张文博[1] 赵紫罡[1] 李文涵 李敏[1] 赵娜[2] ZHANG Wen-bo;ZHAO Zi-gang;LI Wen-han;LI Min;ZHAO Na(Baotou Tumor Hospital,Baotou,Inner Mongolia O14030,China;不详)

机构地区：[1]包头市肿瘤医院胃肠肝胆外科,内蒙古包头014030 [2]内蒙古科技大学包头医学院第一附属医院医疗保健中心老年病科,内蒙古包头014010

出　　处：《中华医院感染学杂志》2023年第17期2656-2660,共5页Chinese Journal of Nosocomiology

基　　金：内蒙古自治区科研基金资助项目(2021KJ0341)。

摘　　要：目的构建结直肠癌术后腹腔感染的风险预测模型,分析Hippo信号通路基因表达。方法回顾性收集2018年1月-2019年10月包头市肿瘤医院收治的443例结直肠癌患者临床资料,根据患者术后是否发生腹腔感染分为感染组55例和未感染组388例。入院后收集患者临床资料,检测Hippo信号通路基因表达情况,多因素Logistic回归分析结直肠癌患者术后腹腔感染的危险因素,并构建预测模型,采用受试者工作特征(ROC)曲线分析模型的预测价值。结果多因素Logistic回归分析结果显示,合并糖尿病、手术时间较长、Yes相关蛋白质(Yap)信使核糖核酸(mRNA)、转录共激活因子PDZ结合基序(taZ)mRNA、哺乳动物STE20样蛋白激酶1(mst1)mRNA水平较高均为结直肠癌术后腹腔感染的独立危险因素(P<0.05)。ROC曲线分析结果显示,根据上述危险因素构建的模型预测结直肠癌患者术后腹腔感染的曲线下面积(AUC)为0.846,敏感度为80.00%,特异度为76.29%。结论结直肠癌术后腹腔感染的独立危险因素包括合并糖尿病、手术时间较长、Yap mRNA、taZ mRNA、mst1 mRNA水平较高,通过危险因素构建的模型对结直肠癌术后腹腔感染的预测价值较高。OBJECTIVE To establish the risk prediction model for postoperative abdominal infection in the patients with colorectal cancer and analyze the expressions of Hippo signaling pathway genes.METHODS The clinical data were retrospectively collected from 442 colorectal cancer patients who were treated in Baotou Tumor Hospital from Jan 2018 to Oct 2019.The patients were divided into the infection group with 55 cases and the no infection group with 388 cases according to the status of postoperative abdominal infection.The clinical data were collected from the patients after the admission,the expression levels of Hippo signaling pathway genes were detected.Multivariate logistic regression analysis was performed for the risk factors for the postoperative abdominal infection,the prediction model was established,and the predictive value of the model was analyzed by means of receiver operating characteristic(ROC)curve.RESULTS The result of multivariate logistic regression analysis showed that complication with diabetes mellitus,long operation duration and high levels of Yes-associated protein(Yap)messenger ribonucleic acid(mRNA),transcriptional coactivator with PDZ-binding motif(taZ)mRNA and mammalian STE20-like protein kinasel(mst1)mRNA were the independent risk factors for the postoperative abdominal infection in the colorectal cancer patients(P<0.05).ROC curve analysis indicated that the area under curve(AUC)of the model established based on the above risk factors was 0.846 in prediction of the postoperative abdominal infection,with the sensitivity 80.00%,the specificity 76.29%.CONCLUSION The independent risk factors for the postoperative abdominal infection include the complication with diabetes mellitus,long operation duration and the high levels of Yap mRNA,taZ mRNA and mst1 mRNA.The value of the model established based on the risk factors is high in prediction of the postoperative abdominal infection in the colorectal cancer patients.

关 键 词：结直肠癌 腹腔感染 预测模型 Hippo信号通路 基因 预后 

分 类 号：R735.3[医药卫生—肿瘤]