电针百会、神庭对脂多糖诱导的抑郁样小鼠行为及海马NOD样受体蛋白3炎症小体的影响  

Efficacy of electroacupunctureat Baihui(GV20)and Shenting(GV24)on lipopolysaccharide-induced depression-like behavior in mice and its effect on NOD-like receptor protein 3inflammasome in hippocampal region

作　　者：杨柳笛 李红蕾 YANG Liudi;LI Honglei(Medical College of Xi'an International University,Xi'an,Shaanxi 710077;Engineering Research Center of Personalized Anti-aging Health Product Development and Transformation,Universities of Shaanxi Province Xi'an,Xi'an,Shaanxi 710077)

机构地区：[1]西安外事学院医学院,陕西西安710077 [2]西安外事学院精准抗衰健康产品研发与转化陕西省高校工程研究中心,陕西西安710077

出　　处：《河北中医》2023年第10期1693-1697,1702,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：陕西省教育厅科学研究计划项目(编号:20JK0862)。

摘　　要：目的观察电针百会、神庭对脂多糖(LPS)诱导的抑郁样小鼠行为及NOD样受体蛋白3炎症小体(NLRP3)的影响。方法将32只雄性C57BL/6小鼠随机分为对照组、模型组、电针组和氟西汀组,每组8只。模型组、电针组、氟西汀组小鼠每日予腹腔注射脂多糖(LPS)建立急性抑郁小鼠模型,连续5天。在造模同时,电针组每日予百会、神庭穴电针治疗,氟西汀组每日予氟西汀20 mg/kg灌胃,对照组、模型组小鼠每日予等容积0.9%氯化钠注射液灌胃,连续5天。干预结束后采用旷场实验、强迫游泳实验进行行为学测试,评估各组小鼠行为学变化。采用蛋白免疫印迹法(Western Blot)检测海马组织NLRP3、半胱天冬酶-1(Caspase-1)、白细胞介素1β(IL-1β)的蛋白表达。结果旷场实验结果显示,各组小鼠的总运动总路程比较差异无统计学意义(P<0.05)。与对照组比较,模型组小鼠中央区域停留时间明显缩短(P<0.05);与模型组比较,电针组、氟西汀组小鼠中央区域停留时间均明显延长(P<0.05);与电针组比较,氟西汀组小鼠中央区域停留时间延长(P<0.05)。强迫游泳实验结果显示,与对照组比较,模型组小鼠不动时间明显延长(P<0.05);与模型组比较,电针组、氟西汀组小鼠不动时间均缩短(P<0.05);与电针组比较,氟西汀组小鼠不动时间缩短(P<0.05)。Western Blot结果显示,与对照组比较,模型组海马NLRP3、Caspase-1、IL-1β蛋白表达均升高(P<0.05);与模型组比较,电针组、氟西汀组NLRP3、Caspase-1、IL-1β蛋白表达均降低(P<0.05);电针组与氟西汀组组间比较差异无统计学意义(P>0.05)。结论电针治疗可显著改善LPS诱导的小鼠抑郁样行为,其作用机制可能与抑制NLRP3信号通路的活化有关。Objective To observe the efficacy of electroacupuncture(EA)at Baihui(GV20)and Shenting(GV24)on lipopolysaccharide(LPS)-induced depression-like behavior in mice and its effect on NOD-like receptor protein 3(NLRP3)inflammasome in hippocampal region.Methods Thirty-two male C57BL/6 mice were randomly divided into 4 groups:control group,model group,EA group and fluoxetine group,with 8 mice in each group.In addition to the control group,mice in the other three groups were intraperitoneally injected with LPS daily to establish acute depression mice model for 5 days.Meanwhile,the EA group received EA at Baihui(GV20)and Shenting(GV24)daily,the fluoxetine group received 20 mg/kg fluoxetine by gavage daily,and the control and model groups received an equal volume of 0.9%sodium chloride injection by gavage.Behavioral tests were performed to evaluate the behavioral changes of mice through open field test(OFT)and forced swimming test(FST).The expression of NLRP3 inflammasome in hippocampal region,caspase-1,and interleukin 1β(IL-1β)was detected using Western Blot.Results The results of OFT showed that the total movement distance of mice in each group was comparable(P>0.05).Compared with the control group,the time spent in the center in the model group was significantly shortened(P<0.05),which in the EA group was significantly longer than the model group(P<0.05),and the fluoxetine group was significantly longer than the EA group(P<0.05).The results of FST showed that the immobility time in the model group was significantly longer than that of the control group(P<0.05);in the comparison of the other three groups except the control group,the immobility time was the shortest in the fluoxetine group,followed by the EA group,and the model group(all P<0.05).Western Blot results showed that the expressions of NLRP3,Caspase-1,and IL-1βin the model group were significantly higher than those in the control group(P<0.05),which in the EA group and the fluoxetine group were significantly lower than those in the model group(P<0.05),and wit

关 键 词：百会 神庭 抑郁症 小鼠 电针 行为 NOD样受体蛋白3炎症小体 

分 类 号：R749.42[医药卫生—神经病学与精神病学]