约束视距下照度诱导近视动物模型的研究  

Study of myopic animal model induced by illuminance under constrained visual range

作　　者：张凯雪 方文卿[1,2] 陈娟 张友铭[1] 杨超普 金涵 陈太阳[1] 黄三晓 周鹏辉 Zhang Kaixue;Fang Wenqing;Chen Juan;Zhang Youming;Yang Chaopu;Jin Han;Chen Taiyang;Huang Sanxiao;Zhou Penghui(School of Materials Science and Engineering,Nanchang University,Nanchang 330031,China;National Engineering Technology Research Center for LED on Silicon Substrate,Nanchang 330047,China;Ophthalmic Center of Jiangxi Provincial People's Hospital,Nanchang 330006,China;School of Chemical Engineering and Modern Materials,Shangluo University,Shangluo Shaanxi 726000,China)

机构地区：[1]南昌大学材料科学与工程学院,330031 [2]南昌大学国家硅基LED工程技术研究中心,330047 [3]江西省人民医院眼科中心,南昌330006 [4]商洛学院化学工程与现代材料学院,陕西商洛726000

出　　处：《眼科》2023年第4期294-298,共5页Ophthalmology in China

基　　金：国家自然科学基金(61864008);国家重点研发计划项目(2017YFB0403700)。

摘　　要：目的 研究约束视距下不同照度对小鸡眼轴发育的影响。设计实验研究。研究对象60只10日龄小鸡。方法随机分为5组,每组12只,其中一组为对照组,其余为实验组。利用小鸡对食物的贪婪及趋光性,较自然地约束小鸡视距。4个实验组小鸡分别饲养在4套自制的近视诱导装置中,装置能随小鸡生长,将其视距始终约束在约5 cm之内。照度分别设为0.5 Lx、5Lx、50 Lx、500 Lx;对照组不约束视距,在靠窗的自然光环境下饲养。在暗室非局麻条件下,分别用A超测量眼轴长度,检影镜测量屈光度;电子称测量体重,计算各实验组眼轴的异常增幅,并关联此值与屈光度的关系。主要指标眼轴长度、眼轴增幅、屈光度。结果对照组眼轴长度和体重成线性关系(R2=0.89);鸡龄50天时0.5 Lx、5 Lx、50 Lx、500 Lx组的眼轴异常增幅分别为0.60mm、0.30 mm、0.75 mm、0.80 mm;5 Lx比0.5 Lx组、50 Lx组、500 Lx组的眼轴异常增幅明显小(P=0.033、0.001、0.003)。屈光度与眼轴的异常增幅存在线性关系(R2=0.74)。结论本模型能显著诱导小鸡轴性近视。照度对眼轴异常增幅影响明显,5 Lx下的眼轴异常增幅最小,500 Lx组最大。Objective To study the effect of different illumination on the development of axial length in chicks with visual distance restriction.Design Experiment study.Participants Sixty 10-day-old chicks.Methods Chicks were randomly divided into 5 groups with 12 chicks in each group.One group was the control group and the others were the experimental groups.Using the greedy and phototaxis of the chicken to food,the visual distance of the chicken was more naturally restricted.Four groups of chickens were raised in four sets of self-made myopia induction devices,which could keep the visual distance within about 5 cm with the growth of chicks.The illuminances of the experimental groups were set at 0.5 Lx,5 Lx,50 Lx,and 500 Lx,respectively.The control group was reared in the natural light environment near the window without restricting the visual distance.When the chickens were 50 days old,the axial length of the eyes was measured by A-scan.The refraction was measured by retinoscope in the dark room and under non-local anesthesia.The weight was measured by electronic scale and the abnormal growth of axial length was calculated.The relationship between this value and diopter was analyzed.Main Outcome Measured Axial length,axial increase,diopter.Results There was a linear relationship between axial length and body weight in the control group(R2=0.89).When the chicken were 50 days old,the abnormal axial growth of 0.5 Lx,5 Lx,50 Lx and 500 Lx groups were 0.60 mm,0.30 mm,0.75 mm and 0.80 mm,respectively.There were significant differences in the increase of axial length among 5 Lx group and 0.5 Lx group,50 Lx group,500 Lx group(P=0.033,0.001,0.003).There was a linear proportional relationship between diopter and abnormal axial growth(R2=0.74).Conclusion The model can significantly induce axial myopia in chicks.Illumination had a significant effect on the increase of abnormal axial growth.The increase of abnormal axial growth was the smallest at 5 Lx and the largest at 500 Lx.

关 键 词：近视模型 照度 形觉剥夺性近视 

分 类 号：R778.11[医药卫生—眼科] R-332[医药卫生—临床医学]