p150glued对脊髓运动神经元上谷氨酸能突触输入的调控  

Effects of p150glued on glutamatergic synaptic input in spinal motor neurons of mice

作　　者：郑佳音 杨璇 彭宇 丁思妍 王昕宇 于佳[1] Zheng Jiayin;Yang Xuan;Peng Yu;Ding Siyan;Wang Xinyu;Yu Jia(Institute of Geriatrics and Rehabilitation,Beijing Geriatric Hospital,Beijing 100095,China)

机构地区：[1]北京老年医院老年医学基础研究中心,北京100095

出　　处：《脑与神经疾病杂志》2023年第9期556-561,共6页Journal of Brain and Nervous Diseases

基　　金：北京市自然科学基金资助项目(7202077);国家自然科学基金资助项目(82071438);北京市百千万人才工程资助项目(2017002);北京老年医院老年医学科研专项(2020bjlnyy-自-3)。

摘　　要：目的 探讨运动神经元病相关蛋白p150glued在脊髓前角的亚细胞定位及其对小鼠脊髓运动神经元上谷氨酸能突触输入的调控作用。方法 通过免疫荧光染色技术观察p150glued在小鼠脊髓前角处亚细胞定位情况,以及对照组Ctrl小鼠和p150glued条件性敲除cKO小鼠脊髓运动神经元上谷氨酸能突触前标记物VGLUT1含量变化情况。结果 在小鼠脊髓前角,p150glued的强阳性信号与VGLUT1存在共定位。在6月龄cKO小鼠中进一步发现,p150glued缺失的脊髓运动神经元膜上VGLUT1阳性点状信号多于Ctrl小鼠。结论 p150glued富集于兴奋性谷氨酸能突触前,p150glued缺失可增加6月龄小鼠脊髓运动神经元上谷氨酸能突触输入。Objective To investigate the subcellular localization of motor neuron disease associated protein p150glued and its effects on glutamatergic synaptic input in spinal motor neurons of mice.Methods The subcellular localization of p15Oglued and the levels of VGLUT1 was observed by using immunofluorescence staining in the spinal cord of Ctrl and cKO mice.Results Co-localization of p150glued and VGLUT1 was observed in the anterior horn of spinal cord of mice.Compared to Ctrl mice,the levels of VGLUT1 were increased in the spinal motor neurons of cKO mice at 6 months.Conclusion p150glued enriched in excitatory glutamatergic presynaptic,and loss-of-function of p150glued in mice spinal motor neurons causes glutamatergic synaptic input increased.

关 键 词：p150glued 运动神经元病 谷氨酸能突触前 VGLUT1 脊髓运动神经元 

分 类 号：R744[医药卫生—神经病学与精神病学]