好氧微生物降解林丹过程中碳-氯二维稳定同位素的分馏特性  

Fractionation characteristics of two-dimensional stable isotopes of carbonchlorine in the process of Lindane degradation by aerobic microorganisms

作　　者：李洁[1] 张新雨 李晓红 蒋中伟 刘峰[4] 单永平 刘亚青 LI Jie;ZHANG Xinyu;LI Xiaohong;JIANG Zhongwei;LIU Feng;SHAN Yongping;LIU Yaqing(Nanning College for Vocational Technology,Nanning 530000,China;College of Light Industry&Food Engineering,Guangxi University,Nanning 530004,China;Guangxi Modern Logistics Group,Nanning 530299,China;Research Center for Eco-Environmental Science,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]南宁职业技术学院,南宁530000 [2]广西大学轻工与食品工程学院,南宁530004 [3]广西现代物流集团有限公司,南宁530299 [4]中国科学院生态环境研究中心,北京100085

出　　处：《环境工程学报》2023年第7期2361-2368,共8页Chinese Journal of Environmental Engineering

基　　金：广西自然科学基金资助(任务书编号:2021GXNSFBA196092);广西科技基地和人才专项资助(任务书编号:AD22080067);国家自然科学基金资助项目(52270144);中国博士后科学基金资助项目(2022M713300)。

摘　　要：六氯环己烷(HCH)是一种持久性污染物,环境存量较大时会造成污染,定量研究其在环境中的迁移转化过程具有重要意义。研究了3种好氧微生物Sphingobium quisquiliarum P25、Sphingobium ummariense RL-3和Sphingobium sp.F2降解γ-HCH(林丹)的过程,应用单体稳定同位素分析技术(CSIA)探究了不同微生物对HCH的降解效果,及其对HCH转化与迁移行为的影响。结果表明:1)3种微生物降解γ-HCH的过程符合一级反应动力学原理;2)降解过程中碳分馏系数(ε_(C))分别为-(4.3±0.4)‰,-(1.6±0.1)‰和-(5.7±0.5)‰,氯同位素分馏系数(ε_(Cl))分别为-(1.1±0.6)‰,-(1.5±0.2)‰和-(1.5±0.4)‰;3)二维同位素分馏系数(Λ_(C-Cl))分别为(3.0±0.3),(1.1±0.1)和(2.7±0.2)。本研究结果可为应用单体稳定同位素分析技术解析复杂环境中HCH的迁移转化过程并评估HCH污染场地修复效果提供参考。Hexachlorocyclohexane(HCH)is a persistent pollutant that causes pollution in large quantities in the environment,it is very important to quantitatively study its migration and transformation process in the environment.The degradation process ofγ-HCH(Lindan)by three aerobic microorganisms,Sphingobium quisquiliarum P25,Sphingobium ummariense RL-3 and Sphingobium sp.F2 were studied.Monomer stable isotope analysis(CSIA)was used to investigate the degradation of HCH by different microorganisms and their effects on the transformation and migration of HCH.The results showed that the degradation ofγ-HCH by three kinds of microorganisms was consistent with the principle of first-order reaction kinetics.Carbon fractionation coefficient(ε_(C))was−(4.3±0.4)‰,−(1.6±0.1)‰and−(5.7±0.5)‰,respectively.Chlorine isotope fractionation coefficient(ε_(Cl))was−(1.1±0.6)‰,−(1.5±0.2)‰,and−(1.5±0.4)‰,respectively.Two-dimensional isotope fractionation coefficients(Λ_(C-Cl))was(3.0±0.3),(1.1±0.1)and(2.7±0.2),respectively,The results of this study can provide a reference for the application of monomer stable isotope analysis technology to analyze the migration and transformation process of HCH in complex environments and evaluate the restoration effect of HCH contaminated sites.

关 键 词：同位素分馏 好氧微生物 六氯环己烷(六六六) 单体稳定同位素分析技术(CSIA) 

分 类 号：X172[环境科学与工程—环境科学]