叶菜型甘薯叶绿体基因组及其特征分析  

Analysis of Chloroplast Genomic Characteristics of Leafy Sweetpotato

作　　者：李国良[1] 张鸿[1] 林赵淼[1] 许泳清[1] 许国春 李华伟[1] 纪荣昌[1] 罗文彬[1] 邱永祥[1] 邱思鑫[1] 汤浩[1] LI Guoliang;ZHANG Hong;LIN Zhaomiao;XU Yongqing;XU Guochun;LI Huawei;JI Rongchang;LUO Wenbin;QIU Yongxiang;QIU Sixin;TANG Hao(Institute of Crop Sciences,Fujian Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture and Rural Affairs,Fuzhou 350013,China)

机构地区：[1]福建省农业科学院作物研究所/农业农村部南方薯类观测实验站,福州350013

出　　处：《西南大学学报（自然科学版）》2023年第10期43-53,共11页Journal of Southwest University(Natural Science Edition)

基　　金：财政部和农业农村部国家现代农业产业技术体系项目(CARS-10-B14);福建省人民政府-中国农业科学院农业高质量发展超越“5511”协同创新工程项目(KXXYJBG0039);福建省属公益类科研院所基本科研专项(2021R1031011)。

摘　　要：为探明叶菜型甘薯叶绿体基因组特征及其与番薯属植物的亲缘关系,以叶菜型甘薯‘福菜薯18号’为试验材料,利用BGISEQ-500平台和Oxford Nanopore Technologies单分子测序技术对全基因组进行建库测序,并组装其叶绿体基因组.结果显示:叶菜型甘薯叶绿体基因组全长161387 bp,具有典型的环状四分体结构,其大单拷贝区(large single copy,LSC)、小单拷贝区(small single copy,SSC)和2个反向重复序列(inverted repeat,IR)的长度分别为87597,12052和30869 bp.注释共得到132个基因,包含87个蛋白编码基因,8个rRNA基因,37个tRNA基因.在叶菜型甘薯叶绿体基因组中共搜索到54个SSR位点,其中单核苷酸、二核苷酸、三核苷酸、四核苷酸、五核苷酸和六核苷酸重复基序个数分别为32,4,3,11,2和2个.系统进化分析表明,叶菜型甘薯与甘薯四倍体野生种Ipomoea tabascana和二倍体野生种Ipomoea trifida具有较近的亲缘关系.In order to find out the characteristics of chloroplast genome of leafy sweetpotato and the genetic relationship of the genus Ipomoea,the whole chloroplast genome of leafy sweetpotato‘Fucaishu18’was sequenced and assembled using the BGISEQ 500 platform and single molecule sequencing technology of Oxford Nanopore Technologies.The results showed that the leafy sweetpotato chloroplast genome was 161387 bp in length,with a typical quadripartite structure of circular molecule,including a pair of inverted repeats(IRA and IRB)of 30869 bp that were separated by large and small single copy regions(LSC and SSC)of 87596 bp and 12052 bp,respectively.A total of 132 genes were annotated,including 87 protein-coding genes,8 rRNA genes,and 37 tRNA genes.We found 54 SSR loci in the leafy sweetpotato chloroplast genome,of which the number of mononucleotide,dinucleotide,trinucleotide,tetranucleotide,pentanucleotide and hexanucleotide repeat motifs were 32,4,3,11,2 and 2,respectively.The phylogenetic analysis showed that the leafy sweetpotato had a relatively close relationship with the wild tetraploid species Ipomoea tabascana and the wild diploid species Ipomoea trifida.

关 键 词：叶菜型甘薯 叶绿体基因组 SSR 系统发育分析 

分 类 号：S531[农业科学—作物学]