不同基因型甘薯叶片酚类质量分数及PPO,POD活性的差异  

Differences of Leaf Phenolic Substances Content and PPO,POD Activities among Sweetpotato Genotypes

作　　者：冉海榕 李佳欣 傅玉凡[1] 陈培涛 王璐璐 黄雨 宗继锴 罗青青 RAN Hairong;LI Jiaxing;FU Yufan;CHEN Peitao;WANG Lulu;HUANG Yu;ZONG Jikai;LUO Qingqing(School of Life Science,Southwest University/Engineering and Technology Research Center for Sweetpotato of Chongqing,Chongqing 400715,China)

机构地区：[1]西南大学生命科学学院/重庆市甘薯工程技术研究中心,重庆400715

出　　处：《西南大学学报（自然科学版）》2023年第10期54-62,共9页Journal of Southwest University(Natural Science Edition)

基　　金：财政部和农业农村部国家现代农业产业技术体系项目(CARS-10-C20);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0100)。

摘　　要：以86个甘薯基因型的叶片为供试材料,研究其总多酚、绿原酸和干物质的质量分数,多酚氧化酶(PPO)、过氧化物酶(POD)活性,ABTS自由基清除能力在基因型间和不同的色泽与干物质质量分数群体之间的差异及其相关性,以期为甘薯叶片相关育种与利用研究奠定一些基础.结果表明:甘薯叶片总多酚和绿原酸的质量分数、ABTS自由基清除率在基因型间和不同干物质质量分数基因型群体间差异有统计学意义,总多酚和绿原酸的质量分数及其ABTS自由基清除能力与叶片干物质质量分数之间呈显著正相关.PPO,POD活性在基因型间的差异有统计学意义,但与叶片色泽、干物质、总多酚和绿原酸的质量分数以及ABTS自由基清除能力间没有明显的相关性规律.The difference and correlation of the contents of total polyphenols,chlorogenic acid and dry matter,activities of PPO,POD,ABTS+scavenging capacity in leaves among 86 sweetpotato varieties and populations of different color or dry matter content were studied in present paper,in order to provide a theoretical basis for the utilization of sweetpotato leaves and related breeding.The results showed that the content of total polyphenols and chlorogenic acid in leaves and the scavenging rate of ABTS+free radicals were significantly different among the varieties and the populations with different dry matter content,and the contents of total polyphenols and chlorogenic acid and their ABTS+scavenging capacity had significant positive correlation with dry matter content of leaves.The activities of PPO and POD were significantly different among varieties,but not obvious correlated with the leaf color,dry matter content,contents of total polyphenols and chlorogenic acid,ABTS+scavenging capacity.

关 键 词：甘薯叶片 总多酚 绿原酸 ABTS自由基清除能力 多酚氧化酶 过氧化物酶 

分 类 号：S531[农业科学—作物学]