甘薯叶片绿原酸及其抗氧化活性的基因型差异  

Genotypic Differences of Chlorogenic Acids and Theirs Antioxidant Activities in Sweetpotato Leaves

作　　者：罗青青 陈培涛 宗继锴 傅玉凡[1] 赵腾飞 徐元江 高纪龙 秦瑞华 LUO Qingqing;CHEN Peitao;ZONG Jikai;FU Yufan;ZHAO Tengfei;XU Yuanjiang;GAO Jilong;QIN Ruihua(School of Life Science,Southwest University/Engineering and Technology Research Center for Sweetpotato of Chongqing,Chongqing 400715,China;Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)

机构地区：[1]西南大学生命科学学院/重庆市甘薯工程技术研究中心,重庆400715 [2]重庆市中药研究院,重庆400065

出　　处：《西南大学学报（自然科学版）》2023年第10期63-71,共9页Journal of Southwest University(Natural Science Edition)

基　　金：财政部和农业农村部国家现代农业产业技术体系项目(CARS-10-SYZ15);重庆市农业农村委薯类科企联合体项目。

摘　　要：在建立高效液相色谱法(HPLC)检测方法的基础上,对50个甘薯基因型叶片的6种绿原酸组分的质量分数和叶片乙醇提取物的ABTS,DPPH,FRAP抗氧化能力进行了测定和分析,以期探究甘薯叶片绿原酸组分质量分数及其抗氧化能力在基因型之间的差异,筛选叶富含绿原酸和高抗氧化活性的种质资源.结果表明:甘薯叶片均含有3-CQA,4-CQA,5-CQA,3,4-diCQA,3,5-diCQA和4,5-diCQA 6种组分,6种组分的总质量分数每100 g干物质中含310.34~5033.86 mg,变异系数为50.17%,平均值为2398.48 mg,各个组分的质量分数及其总和在基因型间差异有统计学意义.3,5-diCQA,3,4-diCQA和3-CQA共3种绿原酸组分是50个基因型叶片绿原酸的主要成分,其质量分数在总质量分数中的占比平均值分别为35.36%,22.98%和21.54%.叶片绿原酸提取物的ABTS,DPPH自由基清除能力和FARP铁离子还原能力在基因型间差异有统计学意义,且3种抗氧化能力均与绿原酸各组分质量分数和总质量分数呈极显著正相关.通过研究与分析,筛选出叶片富含绿原酸、高抗氧化活性的18-11-4,161837,170407,18-6-33,18-11-5优异种质资源,结果为甘薯叶片绿原酸进一步研究以及相关育种工作奠定了一定基础.On the basis of establishing HPLC method for determination of the content of 6 chlorogenic acid components in sweetpotao leaves,the contents of 6 chlorogenic acid components and the antioxidant capacities of ABTS,DPPH and FRAP of ethanolic extracts from leaves of 50 sweetpotato genotypes were detected and analyzed in present study,in order to investigate the differences in chlorogenic acid content and antioxidant capacity among the genotypes,and to select germplasm resources rich in chlorogenic acid and high antioxidant activity.The results showed that all sweetpotato genotypes contained 3-CQA,4-CQA,5-CQA,3,4-diCQA,3,5-diCQA and 4,5-diCQA in their leaves.The results showed that all sweetpotato leaves contained 6 fractions of 3-CQA,4-CQA,5-CQA,3,4-diCQA,3,5-diCQA and 4,5-diCQA,and the total content of the 6 fractions ranged from 310.34 to 5033.86 mg/100 g DW with a coefficient of variation of 50.17%and a mean value of 2398.48 mg/100 g DW.Three chlorogenic acid fractions,3,5-diCQA,3,4-diCQA and 3-CQA,were the major components of chlorogenic acid in the leaves of the 50 genotypes,with mean contents of 35.36%,22.98%and 21.54%in the total content,respectively.The ABTS,DPPH radical scavenging ability and FARP ferric ion reducing ability of chlorogenic acid extracts from leaves were highly significantly different among the genotypes,and all three antioxidant abilities were highly significantly positively-correlated with the content of each component and the total content of chlorogenic acids.“18-11-4”“161837”“170407”“18-6-33”and“18-11-5”with high chlorogenic acid and antioxidant activity were selected.The results of this study laid the foundation for further research on sweet potato chlorogenic acid in leaves and related breeding work.

关 键 词：甘薯 叶片 绿原酸 抗氧化活性 种质资源 

分 类 号：S531[农业科学—作物学]