活性氧介导非热大气压等离子体杀伤肺癌细胞的机制  

The killing mechanism of lung cancer cells by nonthermal atmospheric pressure plasma mediated by ROS

作　　者：周袁媛 刘芳 陈金武 刘磊[2] 宋文成[2,3,5] 储焰南[2,3] ZHOU Yuanyuan;LIU Fang;CHEN Jinwu;LIU Lei;SONG Wencheng;CHU Yannan(Institute of Physical Science and Information Technology,Anhui University,Hefei 230601,China;Anhui Province Key Laboratory of Medical Physics and Technology,Institute of Health&Medical Technology,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Hefei Cancer Hospital,Chinese Academy of Sciences,Hefei 230031,China;School of Biology and Food Engineering,Hefei Normal University,Hefei 230061,China;Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions,Suzhou 215123,China)

机构地区：[1]安徽大学物质科学与信息技术研究院,合肥230601 [2]中国科学院合肥物质科学研究院安徽省医学物理与技术重点实验室健康与医学技术研究所,合肥230031 [3]中国科学院合肥肿瘤医院,合肥230031 [4]合肥师范学院生物与食品工程学院,合肥230061 [5]江苏高校放射医学协同创新中心,苏州215123

出　　处：《生物学杂志》2023年第5期1-5,共5页Journal of Biology

基　　金：国家自然科学基金项目(21876179,22142007);中国博士后科学基金项目(2020T130117ZX,2020M671914);安徽省博士后基金项目(2020B470);安徽省自然科学基金项目(2008085MC65);安徽省高校自然科学基金项目(KJ2017A935)。

摘　　要：使用非热大气压等离子体(nonthermal atmospheric plasma,NAP)处理肺癌H460细胞,随着NAP作用时间的延长,MTT实验结果显示:NAP可以导致H460活力显著下降,表明NAP能够有效抑制肺癌细胞的生长;JC-1检测实验表明NAP导致H460线粒体膜电位下降,代表着细胞凋亡的发生。进行Western Blot实验,发现NAP可以抑制细胞内蛋白PI3K、AKT、JNK、STAT3的表达,提示NAP对H460的杀伤作用与PI3K/AKT和JNK/STAT3/AKT的表达有关。另外,在培养基中加入活性氧(ROS)清除剂NAC,可以有效消除NAP对H460的影响,表明NAP对肺癌细胞H460的杀伤由ROS介导,研究为NAP应用于肺癌治疗的可行性提供理论依据。The nonthermal atmospheric plasma(NAP)was used to treat lung cancer cells H460.With the extension of NAP treatment time,MTT assay showed that NAP could decrease the cell viability of H460 significantly,JC-1 assay showed that NAP decreased the mitochondrial membrane potential of H460,which represented the occurrence of cell apoptosis.Western Blot experiment showed that NAP inhibited the expression of PI3K,AKT,JNK and STAT3,demonstrating the killing effect of NAP related to the expression of PI3K/AKT and JNK/STAT3/AKT.Besides,the addition of ROS scavenger NAC effectively eliminated the damage caused by NAP,suggesting that the killing of lung cancer H460 cells by NAP were mediated by ROS,which provided theoretical basis for the feasibility of NAP in the treatment of lung cancer.

关 键 词：非热大气压等离子体 肺癌 H460细胞 活性氧 细胞凋亡 

分 类 号：Q274[生物学—细胞生物学] Q6[医药卫生—肿瘤] R730.5[医药卫生—临床医学] R734.2