云南地区宫颈癌癌前病变患者HPV 56的分子流行病学调查  

Molecular Epidemiological Investigation of HPV 56 in Precancerous Cervical Cancer Patients in Yunnan Province

作　　者：周紫云 王莹莹 严志凌[3] 赵凌锋 杨希[3] 许金美[3] 张芍[3] ZHOU Ziyun;WANG Yingying;YAN Zhiling;ZHAO Lingfeng;YANG Xi;XU Jinmei;ZHANG Shao(Center for Disease Control and Prevention,Qujing City,Qujing Yunnan 655000;School of Basic Medicine,Kunming Medical University,Kunming Yunnan 650500;Dept.of Gynaecologic Oncology,The 3rd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650118,China)

机构地区：[1]曲靖市疾病预防控制中心,云南曲靖655000 [2]昆明医科大学基础医学院,云南昆明650500 [3]昆明医科大学第三附属医院妇科,云南昆明650118

出　　处：《昆明医科大学学报》2023年第10期114-121,共8页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202201AY070001-139)。

摘　　要：目的研究单一感染HPV56型的癌前病变患者中HPV56 E6、E7和L1基因变异特征,获取云南地区宫颈癌癌前病变患者中HPV56型分子流行病学数据。方法随机选取300例宫颈癌癌前病变患者,通过HPV分型试剂盒对患者感染HPV的型别进行检测,并对HPV56型感染患者中HPV E6 E7和L1基因进行测序,最后完成对研究对象所感染的HPV56的谱系分布及HPV56 E6、E7和L1基因的遗传变异进行分析。结果研究对象所感染的HPV56病毒主要为A谱系,90%以上为A谱系,约10%为B谱系,HPV56 E6、E7、L1基因中共存在13个变异位点,其中E6基因中6个、E7基因中4个、L1基因中3个。HPV56 E6基因的6个变异位点中有2个为同义突变(T158C(S19S)、G437A(P112P)),4个为非同义突变(A141C(S14R)、A241G(N47S)、A263C(K54N)、G279A(D60N))。HPV56 E7基因的4个变异位点中均为非同义突变(C601A(D10E)、G605A(V12I)、G770C(E67Q)、G802C(Q77H))。HPV56 L1基因的3个变异位点中有2个为同义突变(G6454T(P321P)、A6868C(I459I)),1个为非同义突变(A5867G(N126D))。结论初步获得了云南地区宫颈癌癌前病变患者中HPV56 E6、E7和L1基因变异情况,获得了HPV56 E6、E7和L1基因的分子流行学特征,为进一步分析云南地区宫颈癌癌前病变患者中HPV56 E6、E7和L1基因的分子流行病学特征提供了必要的数据。Objective To study the genetic variation characteristics of HPV56 E6,E7 and L1 in patients with precancerous lesions infected with HPV56,and to obtain the molecular epidemiological data of HPV56 in patients with precancerous lesions of cervical cancer in Yunnan.Methods A total of 300 cervical intraepithelial neoplasia patients were enrolled and HPV genotyping kit was used to detect the types of HPV infection in the patients.The HPV E6,E7,and L1 genes in patients infected with HPV 56 were sequenced.Finally,the lineage distribution of HPV 56 and the genetic variations of HPV 56 E6,E7,and L1 genes in the study subjects were analyzed.Results In this study,the HPV56 virus infected by the study subjects mainly belonged to the A lineage,with more than 90%belonged to the A lineage,and about 10%belonged to the B lineage.There were 13 mutation loci in the E6,E7 and L1 genes of HPV56,including 6 in the E6 gene,4 in the E7 gene and 3 in the L1 gene.Among the 6 mutation sites of HPV56 E6 gene,2 were synonymous mutations(T158C(S19S),G437A(P112P)),and 4 were non-synonymous mutations(A141C(S14R),A241G(N47S),A263C(K54N),G279A(D60N)).The four mutation sites of HPV56 E7 gene were all non-synonymous mutations(C601A(D10E),G605A(V12I),G770C(E67Q),G802C(Q77H)).Among the three mutation sites of HPV56 L1 gene,two were synonymous mutations(G6454T(P321P),A6868C(I459I)),and one was non-synonymous mutation(A5867G(N126D)).Conclusion This study obtained preliminary data on the genetic variations of HPV 56 E6,E7,and L1 genes in CIN patients in Yunnan area,and obtained the molecular epidemiological characteristics of HPV 56 E6,E7,and L1 genes.It provides necessary data for further analysis of the molecular epidemiological characteristics of HPV 56 E6,E7,and L1 genes in CIN patients in Yunnan area.

关 键 词：宫颈癌癌前病变 HPV56 变异 谱系 

分 类 号：R737.3[医药卫生—肿瘤]