One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes  

作　　者：Dagan Zhang Yuze Wang Junqi Zhao Xueqin Li Yuanyang Zhou Sen Wang 

机构地区：[1]Department of Andrology,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China [2]Department of Clinical Laboratory,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,210008,China

出　　处：《Engineered Regeneration》2023年第3期238-244,共7页再生工程（英文）

基　　金：supported by the National Natural Science Foundation of China (82102511),the Natural Science Foundation of Jiangsu (BK20210021);the Research Project of Jiangsu Province Health Committee (M2021031);Clinical Trials from the Affiliated Drum Tower Hospital,Medical School of Nanjing University (2022-LCYJ-PY-05).

摘　　要：Multiplex,rapid and accurate virus quantification plays a great value in biomedical detection.Here,a novel one step,wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored.PhC barcodes were decorated with PDA by self-polymerization of DA,thus this nanocomposite hybridized PhC barcodes facilitated the adsorption of FITC labelled antibodies and quenched itself photolumines-cent,allowing a fast responsive composite platform.In the presence of target analyte,the FITC-labelled detection antibody was released from the surface of PDA decorated microcarrier to specifically bind to the target ana-lyte,thus recovered the photoluminescence.In addition,the PhC microcarrier was enabled to carry out various color barcode for different targets detection though tuning internal periodic structures.Based on these excellent performances of the nanocomposite barcode,this method can not only capture H1N1,H5N1,SARS-CoV-2 si-multaneously with rapid,accuracy but also accomplish multiplex quantification detection with high-sensitivity.Furthermore,our developed platform was also achieved with high-sensitivity and high-specificity through the verification of clinical samples,thus laying out a new avenue for multiplex virus detection in clinical diagnosis.

关 键 词：BARCODE Photonic crystals Wash-free IMMUNOASSAY 

分 类 号：R318[医药卫生—生物医学工程]