Autophagy mediates osteoporotic bone regeneration induced by micro-/nano-structured modification on hydroxyapatite bioceramics  

作　　者：Jinjie Cui Xinran Zhang Liming Cheng Kaili Lin 

机构地区：[1]Department of Oral and Cranio-maxillofacial Surgery,College of Stomatology,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Jiao Tong University,Shanghai 200011,China [2]Shanghai Engineering Research Center of Tooth Restoration and Regeneration,School and Hospital of Stomatology,Tongji University,Shanghai 200072,China [3]Department of Spine Surgery,Tongji Hospital,Tongji University School of Medicine,Shanghai 200065,China [4]Ministry of Education,Key Laboratory of spine and spinal cord injury repair and regeneration(Tongji University),Shanghai,China

出　　处：《Engineered Regeneration》2023年第3期245-256,共12页再生工程（英文）

基　　金：support from the National Natural Science Foundation of China (82072396,32271379);Science and Technology Commission of Shanghai Municipality (21490711700);the Interdisciplinary Program of Shanghai Jiao Tong University (YG2021ZD12).

摘　　要：Osteoporosis(OP)is an age-related disease of bone metabolism,characterized by bone mass loss and bone mi-croarchitecture deterioration,the poor osteogenesis microenvironment of OP caused hardly repairing of the bone defects.As a dynamic process to fuel cellular renovation,autophagy has been proved to play a vital role in regulating cell differentiation and maintaining bone homeostasis.Traditional bone repairing biomaterials are hardly repairing the bone defects under OP pathological microenvironment.Therefore,it is essential to develop-ment novel biomaterials to improve osteoporotic osteogenesis.Compared to biochemical cues,biophysical cues exhibited more advantages in biocompatible and side effects.Herein,inspired by the importance of enhanced au-tophagic response in osteoporotic environment,we intend to utilize the micro-/nano-structured hydroxyapatite(mnHA)bioceramics as the mimic structure of natural bone tissue to regulate autophagic activity in ovariectomy bone mesenchymal stem cells(OVX-BMSCs),finally promote to bone regeneration in OP condition.The results indicated that mnHA bioceramics promoted cell adhesion and osteogenesis of OVX-BMSCs,and enhanced au-tophagy level in OVX-BMSCs.In the calvarial defects of OVX-rats,the mnHA scaffold acquired excellent bone repair effect.According to the current findings,regulating the level of autophagy could be a promising strategy for improve osteoporotic osteogenesis in the future.

关 键 词：Osteoporosis Osteogenesis AUTOPHAGY HYDROXYAPATITE Micro-/nano-structured modification 

分 类 号：R318[医药卫生—生物医学工程]