安徽省稻田千金子对氰氟草酯和噁唑酰草胺的抗性及其靶标分子机制  

Resistance of Chinese sprangletop Leptochloa chinensis to cyhalofop-butyl and metamifop in rice fields in Anhui Province and its possible molecular mechanism

作　　者：韩云静 孟紫为 武健 张勇 周凤艳 Han Yunjing;Meng Ziwei;Wu Jian;Zhang Yong;Zhou Fengyan(Institute of Plant Protection and Agro-Products Safety,Anhui Academy of Agricultural Sciences,Hefei 230031,Anhui Province,China)

机构地区：[1]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230031

出　　处：《植物保护学报》2023年第4期1089-1095,共7页Journal of Plant Protection

基　　金：安徽省农业科技成果转化应用专项(2022ZH001);安徽省农业科学院人才项目(QNYC-202118)。

摘　　要：为明确安徽省稻田杂草千金子Leptochloa chinensis对氰氟草酯和噁唑酰草胺的抗性,从安徽省12个市38个县(市、区)稻区千金子发生较严重的田块中共采集72个千金子种群,采用整株生物测定法检测其对氰氟草酯和噁唑酰草胺的抗性,筛选同时对噁唑酰草胺和氰氟草酯有疑似抗性的千金子种群并测定其对这2种药剂的抗性水平,同时利用分子生物学技术检测千金子高抗种群的乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACCase)基因是否发生抗性位点氨基酸突变。结果显示,在72个千金子种群中,13个千金子种群已对氰氟草酯产生抗性,5个千金子种群已对噁唑酰草胺产生抗性;AH-1、AH-3、AH-7、AH-34和AH-72这5个千金子种群同时高抗氰氟草酯和噁唑酰草胺,其中对氰氟草酯的抗性指数介于21.98~65.52之间,对噁唑酰草胺的抗性指数介于11.76~27.19之间;这5个种群的ACCase基因片段均在2027位点发生氨基酸突变,色氨酸突变为半胱氨酸;此外AH-3和AH-72这2个种群在1999位点也由色氨酸突变为半胱氨酸,表明安徽省千金子已经对氰氟草酯和噁唑酰草胺产生交互抗性,且ACCase基因突变可能是产生抗性的主要原因。To understand the resistance of Chinese sprangletop Leptochloa chinensis to cyhalofop-butyl and metamifop in rice field in Anhui Province,72 populations of L.chinensis were collected from 38 counties(cities,districts)in 12 cities of Anhui Province,and their resistance to cyhalofop-butyl and metamifop was analyzed with the whole-plant bioassay method.The population with putative resistance to cyhalofop-butyl and metamifop were screened and the resistance levels of them were determined.The molecular biological techniques were used to detect the(amino acid)mutation of the acetyl-CoA carboxylase(ACCase)target enzyme gene in the highly resistant population.The results showed that 13 populations were resistant to cyhalofop-butyl,and five to metamifop in the 72 populations of L.chinensis.Five populations(AH-1,AH-3,AH-7,AH-34 and AH-72)showed high resistance to cyhalofop-butyl and metamifop,and the resistance index to cyhalofop-butyl ranged from 21.98 to 65.52,while the resistance indexes to metamifop ranged from 11.76 to 27.19.ACCase gene fragment was mutated at the position 2027 in all five populations,which mutated from tryptophan 2027 to cysteine.In addition,two populations(AH-3 and AH-72)also mutated from tryptophan to cysteine at the site 1999.The results showed that the cross-resistance to cyhalofop-butyl and metamifop had occurred in L.chinensis in Anhui Province,and the mutation of target enzyme gene might be the main cause for the resistance.

关 键 词：千金子 氰氟草酯 噁唑酰草胺 抗性 突变位点 靶标抗性 

分 类 号：S451.21[农业科学—植物保护]