The synbiotic mixture of lactulose and Bacillus coagulans protects intestinal barrier dysfunction and apoptosis in weaned piglets challenged with lipopolysaccharide  

作　　者：Weijiang Zheng Zuyan Zhao Yunnan Yang Liren Ding Wen Yao 

机构地区：[1]College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [2]National Experimental Teaching Center for Animal Science,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [3]Key Lab of Animal Physiology and Biochemistry,Ministry of Agriculture,Nanjing 210095,Jiangsu,China

出　　处：《Journal of Animal Science and Biotechnology》2023年第5期2209-2223,共15页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Key R&D Program of China(2017YFE0114400).

摘　　要：Background Lactulose as an effective prebiotic protects intestinal mucosal injury.Bacillus coagulans is widely used in feed additives because of its ability to promote intestinal health.Our previous study suggests that the combination of lactulose and Bacillus coagulans may be a good candidate as alternative for antibiotic growth promoters.However,the in vivo effects of lactulose and Bacillus coagulans on growth and intestinal health under immune challenge in piglets remains unclear.The objective of this study is to explore the protective effects of synbiotic containing lactu-lose and Bacillus coagulans on the intestinal mucosal injury and barrier dysfunction under immune challenge in weaned piglets.Methods Twenty four weaned piglets were assigned to 4 groups.Piglets in the CON-_(saline)and LPS-_(LPS)group were fed the basal diet,while others were fed either with chlortetracycline(CTC)or synbiotic mixture of lactulose and Bacillus coagulans for 32 d before injection of saline or lipopolysaccharide(LPS).Piglets were sacrificed 4 h after LPS injection to collect samples to determine intestinal morphology,integrity and barrier functions as well as relative genes and proteins.Results Our data showed that no differences were observed in the growth performance of the four test groups.LPS injection induced higher serum diamine oxidase activities,D-lactic acid levels,and endotoxin status,lower villus height and ratio of villus height to crypt depth,greater mRNA and lower protein expression related tight junction in both jejunum and ileum.In addition,a higher apoptosis index,and protein expression of Bax and caspase-3 were also observed in the LPS challenge group.Interestingly,dietary synbiotic mixture with lactulose and Bacillus coagulans protected against LPS-induced intestinal damage,barrier dysfunction and higher apoptosis as well as CTC.Conclusions Our data suggest that dietary supplementation of synbiotic mixture with lactulose and Bacillus coagu-lans showed resilience to LPS-induced intestinal morphological damage,

关 键 词：APOPTOSIS Bacillus coagulans Intestinal barrier function LACTULOSE LPS PIGLETS SYNBIOTIC 

分 类 号：S828.5[农业科学—畜牧学]