Protective effects and microarray-based mechanism of sea cucumber hydrolysates against high-glucose induced nephrotoxicity in mouse glomerulus mesangial cells  

作　　者：Lingxin Geng Jiaojiao Han Jun Zhou Ye Li Tinghong Ming Zhen Zhang Chenyang Lu Xiurong Su 

机构地区：[1]State Key Laboratory for Quality and Safety of Argo-products,School of Marine Science,Ningbo University,Ningbo 315211,China

出　　处：《Journal of Future Foods》2024年第3期233-240,共8页未来食品学报（英文）

基　　金：sponsored by Regional Demonstration Project of Marine Economic Innovation and Development in 2014 and 2016,the National Key Research and Development Program of China(2018YFD0901102);K.C.Wong Magna Fund of Ningbo University.

摘　　要：Diabetic nephropathy(DN)is a common type of end-stage renal disease and glomerular mesangial cells(GMCs)are widely used as a cell model for DN.This study firstly investigated the inhibitory effects of the Apostichopus japonicus and Acaudina leucoprocta hydrolysates on cellular growth under high-glucose treatment,better inhibitory effect of A.japonicus hydrolysate was observed compared to that of A.leucoprocta hydrolysate.Subsequently,the global transcription profiles obtained via microarray analysis showed that 6070 and 7015 genes were identified in the A.japonicus and A.leucoprocta groups compared with the model group,respectively.Among them,transcriptions of the slc30a4,slc35dl,tppp3,tp53inpl,bcl-2,apafl,alox12b and adrala genes were restored from the levels of the model group to those of the control group,contributed to cell mitosis and proliferation in both treatment groups.In addition,other apoptosis-related genes,such as bcl-6,clu,foxo3 and akt,showed opposite trends between two groups,which might cause the difference in inhibitory effect.We preliminarily proposed that the regulation effects of A.japonicus and A.leucoprocta on the genes involved in cellular mitosis,proliferation and apoptosis,might contribute to their inhibitory activity on GMCs under high-glucose environment.

关 键 词：Diabetic nephropathy Apostichopus japonicus Acaudina leucoprocta HYDROLYSATE Rat mesangial cell MICROARRAY 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]