The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC  

作　　者：Rongrong Chen Qianhan Wei Yan Liu Jiankun Li Xuemei Du Yan Chen Jianhua Wang Yunjun Liu 

机构地区：[1]The National Forestry and Grassland Administration Engineering Research Center for Germplasm Innovation and Utilization of Warm-Season Turfgrasses,Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing 210008,Jiangsu,China [2]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China [4]College of Agronomy,Henan Agricultural University,Zhengzhou 450002,Henan,China

出　　处：《The Crop Journal》2023年第5期1368-1379,共12页作物学报（英文版）

基　　金：supported by the Agricultural Science and Technology Innovation Program of CAAS;the Research Program of Sanya Yazhou Bay Science and Technology City (SKJC-2020-02-005);the Natural Science Foundation of Jiangsu Province(BK20200288)。

摘　　要：Although several pentatricopeptide repeat(PPR) proteins are involved in post-transcriptional processing of mitochondrial RNA, it is unclear which specific protein is involved in the RNA editing of ccmC in maize(Zea mays). Here we report the identification of the maize empty pericarp 601(emp601) mutant and the map-based cloning of the Emp601 gene, which encodes an E2-type PPR protein that is targeted to mitochondria. A single-nucleotide deletion in the emp601 mutant caused a frameshift and introduced a premature stop codon into the predicted EMP601. This mutation was associated with reduced accumulation of mitochondrial complex Ⅲ as well as with inhibition of growth and differentiation of basal endosperm transfer layer cells, leading to final degeneration of the embryo and endosperm. We determine that loss of EMP601 function prevents the C-to-U RNA editing of the mitochondrial transcript ccmC at position 358.EMP601 binds to the ccmC transcript and directly interacts with Multiple organellar RNA editing factor 8and may be a component of the plant mitochondrial editosome. We conclude that EMP601 functions in RNA editing of mitochondrial ccmC transcripts and influences mitochondrial function and seed development.

关 键 词：Maize Empty pericarp 601 PPR MITOCHONDRIAL CCMC Seed development 

分 类 号：S513[农业科学—作物学]